The enhancement of PCR amplification by low molecular weight amides
The enhancement of PCR amplification by low molecular weight amides
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DOI：
10.1093/nar/29.11.2377
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发表时间：
2001-06-01
影响因子：
14.9
通讯作者：
Schutt, CE
中科院分区：
文献类型：
--
作者：
Chakrabarti, R;Schutt, CE
通过聚合酶链式反应(PCR)扩增DNA靶标通常需要费力的优化工作。在这方面，已发现使用某些有机化学品，如二甲基亚砜、聚乙二醇、甜菜碱和甲酰胺作为助溶剂是非常有帮助的。不幸的是，人们对使这些添加剂有效的确切结构特征知之甚少，因此，目前已知的增强聚合酶链式反应的此类化学物质的数量有限。为了解决这些问题，我们决定将重点放在甲酰胺上，并作为一类进行了一项广泛的研究，以了解酰胺结构中取代基的变化如何影响其对聚合酶链式反应的影响，我们在这里描述了这项研究的结果，涉及11种不同的酰胺，并提供了这组添加剂中结构-活性关系的内聚性图景。我们发现其中几个酰胺类化合物非常有效，并介绍了它们作为新型的聚合酶链式反应增强剂。
Amplification of a DNA target by the polymerase chain reaction (PCR) often requires laborious optimization efforts. In this regard, the use of certain organic chemicals such as dimethyl sulfoxide, polyethylene glycol, betaine and formamide as cosolvents has been found to be very helpful. Unfortunately, very little is known about the precise structural features that make these additives effective and, accordingly, the number of such chemicals currently known to enhance PCR is limited. In order to address these issues, we decided to focus on formamide and undertook an extensive study of low molecular weight amides as a class to see how changing the substituents in the amide structure influences its effect on PCR, We describe here the results of this study, which involved 11 different amides, and present observations that provide a cohesive picture of structure-activity relations in this group of additives. We found several of these amides to be exceptionally effective and introduce them as novel PCR enhancers.