Selective effects of anti-Ia serum and complement treatment upon polyclonal B lymphocyte responses to dextran sulfate and lipopolysaccharide.
Selective effects of anti-Ia serum and complement treatment upon polyclonal B lymphocyte responses to dextran sulfate and lipopolysaccharide.
复制标题
抗 Ia 血清和补体治疗对多克隆 B 淋巴细胞对硫酸葡聚糖和脂多糖反应的选择性影响。
DOI：
10.4049/jimmunol.123.5.2298
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发表时间：
1979
影响因子：
4.4
通讯作者：
D. Shreffler
中科院分区：
文献类型：
--
作者：
P. H. Bick;D. Shreffler
B淋巴细胞亚群在表达Ia异体抗原和对胸腺非依赖性抗原的多克隆反应性方面存在差异。我们已经证明，多克隆B细胞抗体对硫酸葡聚糖的反应对去除ia阳性细胞的敏感性低于对LPS的反应。这是一个一致的发现，无论同种抗体和补体(C)预处理是针对携带由整个I区编码的Ia抗原的细胞，还是由I- a或I- e亚区编码的细胞。在使用高抗体的葡聚糖硫酸盐敏感人群中似乎存在异质性；在抗体和c介导的细胞选择过程中，细胞比例会抑制增殖反应，但不会抑制抗体反应。这一结果可能表明Ia抗原在葡聚糖硫酸盐敏感的B细胞上的差异表达，这些B细胞通过增殖反应与那些产生抗体的细胞。另外，对葡聚糖硫酸盐的增殖反应可能比多克隆抗体反应更依赖于ia阳性的辅助细胞。
Subpopulations of B lymphocytes have been shown to vary in their expression of Ia alloantigens and polyclonal responsiveness to thymic independent antigens. We have demonstrated that the polyclonal B cell antibody response to dextran sulfate is less sensitive to removal of Ia-positive cells than is the response to LPS. This is a consistent finding whether alloantibody and complement (C) pretreatment is directed toward cells bearing Ia antigens coded for by the entire I region or by the I-A or I-E subregions. Heterogeneity appears to exist within the dextran sulfate-sensitive population in that using high antibody; cell ratios during antibody and C-mediated cell selection results in an inhibition of the proliferative but not the antibody response. This result may indicate a differential expression of Ia antigens on dextran sulfate-sensitive B cells that respond by proliferation versus those cells that produce antibody. Alternatively, proliferative responses to dextran sulfate may be more dependent upon Ia-positive accessory cells than is the polyclonal antibody response.