Differential expression of CD 146 in tissues and endothelial cells derived from infantile haemangioma and normal human skin
Differential expression of CD 146 in tissues and endothelial cells derived from infantile haemangioma and normal human skin
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DOI：
10.1002/path.1443
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发表时间：
2003-10-01
影响因子：
7.3
通讯作者：
Olsen, BR
中科院分区：
文献类型：
--
作者：
Li, Q;Yu, Y;Olsen, BR
血管瘤是最常见的内皮源性肿瘤，发生在4-10%的白人婴儿中。它的特点是出生后第一年迅速增长，然后从1岁到7岁自发消退。细胞表面粘附分子CD 146已被鉴定为内皮细胞标志物。尽管在理解CD 146在正常内皮细胞和肿瘤进展中的功能作用方面取得了进展，但尚未研究其在内皮肿瘤中的表达和可能作用。作为婴儿血管瘤内皮细胞改变的研究的一部分，用几种已知的抗原和内皮细胞标志物进行了差异表达研究。采用免疫组化和流式细胞术分析，培养的人真皮微血管内皮细胞分离的新生儿包皮（HDMEC）与血管瘤组织（HemECs）来源的内皮细胞进行了比较。此外，免疫组化被用来比较血管瘤组织与正常人皮肤。出乎意料的是，通过流式细胞术分析和免疫荧光染色，培养的HemECs显示出比HDMECs显著更低的CD 146水平。使用免疫组化研究，它进一步表明，内皮细胞在所有血管瘤组织，无论肿瘤阶段，显示阴性免疫反应性的CD 146。相反，在内皮层周围的周细胞样细胞中观察到CD 146强阳性染色。这些发现被认为与血管瘤的分子基础有关。此外，抗CD 146的抗体可能用于将血管瘤衍生的内皮细胞与正常内皮细胞和周细胞分离。版权所有（C）2003约翰威利父子有限公司。
Haemangioma is the most common tumour of endothelial origin, occurring in 4-10% of Caucasian infants. It is characterized by rapid growth during the first year of postnatal life, followed by spontaneous regression from 1 to 7 years of age. The cell surface adhesion molecule CD146 has been identified as an endothelial cell marker. Despite advances in understanding the functional role of CD146 in normal endothelial cells and tumour progression, its expression and a possible role in an endothelial tumour have not been studied. As part of an investigation of endothelial cell alterations in infantile haemangioma, differential expression studies were performed with several known antigens and endothelial cell markers. Using immunohistochemical and flow cytometric analyses, cultured human dermal microvascular endothelial cells isolated from newborn foreskin (HDMEC) were compared with endothelial cells derived from haemangioma tissue (HemECs). In addition, immunohistochemistry was used to compare haemangioma tissues with normal human skin. Unexpectedly, cultured HemECs showed a significantly lower level of CD146 than HDMECs by both flow cytometric analysis and immunofluorescence staining. Using immunohistochemical studies, it was further demonstrated that endothelia in all haemangioma tissues, regardless of the tumour phase, showed negative immunoreactivity for CD146. In contrast, strong positive staining for CD146 was observed in the pericyte-like cells that surround the endothelial layers. These findings are believed to be relevant to the molecular basis of haemangioma. Furthermore, it is possible that antibodies against CD146 may be useful for separating haemangioma-derived endothelial cells from normal endothelial cells and pericytes. Copyright (C) 2003 John Wiley Sons, Ltd.