One Argonaute family member, Eif2c2 (Ago2), is essential for development and appears not to be involved in DNA methylation
One Argonaute family member, Eif2c2 (Ago2), is essential for development and appears not to be involved in DNA methylation
复制标题
DOI：
10.1016/j.ygeno.2007.01.004
复制
发表时间：
2007-06-01
期刊：
影响因子：
4.4
通讯作者：
Hatada, Izuho
中科院分区：
文献类型：
--
作者：
Morita, Sumiyo;Horii, Takuro;Hatada, Izuho
为了阐明RNAi在哺乳动物中的表观遗传作用，我们破坏了Eif 2c 2（Ago 2）的基因，该基因在Argonaute家族中作为RNAi的唯一切割器。在小鼠中，Eif 2c 2的破坏导致胚胎在植入阶段后的发育早期死亡。这种表型与以前的报道完全不同，但有点类似于RNAi的另一个重要成分Dicer 1的破坏。我们还表明，Eif 2c 2是不需要的印迹基因，着丝粒重复序列，和Xist的DNA甲基化的维护。这表明Eif 2c 2缺陷小鼠的发育缺陷不是在转录水平引起的，而是在转录后水平通过miRNA-蛋白质复合物引起的。(c)2007年爱思唯尔公司All rights reserved.
To elucidate the epigenetic role of RNAi in mammals, we disrupted the gene for Eif2c2 (Ago2), which works as the sole slicer of RNAi in the Argonaute family. In mice, disruption of Eif2c2 leads to embryonic lethality early in development after the implantation stage. This phenotype is completely different from that in a previous report, but somewhat similar to the disruption of Dicer1, another important component of RNAi. We also show that Eif2c2 is not required for the maintenance of DNA methylation in imprinted genes, centromeric repeats, and Xist. This suggests that developmental defects in the Eif2c2-deficient mouse are caused not at the transcriptional level, but rather at the posttranscriptional level through the miRNA-protein complex. (c) 2007 Elsevier Inc. All rights reserved.