Follicle culture in reproductive toxicology: a tool for in-vitro testing of ovarian function?
Follicle culture in reproductive toxicology: a tool for in-vitro testing of ovarian function?
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DOI：
10.1093/humupd/8.3.243
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发表时间：
2002-05-01
影响因子：
13.3
通讯作者：
Smitz, JEJ
中科院分区：
文献类型：
--
作者：
Cortvrindt, RG;Smitz, JEJ
公众舆论和官方机构非常重视在试验方案中减少、改进和替代实验室动物的使用。在毒理学领域，正在作出重大努力，致力于这一目标。生殖功能的测试目前仍通过体内测试进行，主要是在啮齿动物中进行。在过去，卵泡培养模型被开发用于体外生产成熟卵母细胞，并用于研究体外卵泡发生和卵子发生的过程。这些培养系统可能能够在生育测试中获得一席之地，取代卵巢功能和雌性配子质量测试的体内研究。从一个良好的特征卵泡培养系统的熟练数据表明，这种生物测定法可能是潜在的使用影响卵巢功能和生育能力的外源性物质的体外筛选。
Public opinion and official bodies place great emphasis on the reduction, refinement and replacement of the use of laboratory animals in testing protocols. In the field of toxicology, major efforts are being made to commit to this goal. The testing of reproductive function is currently still performed by in-vivo tests, mainly in rodents. In the past, follicle culture models were developed for the in-vitro production of mature oocytes and used to study the process of folliculogenesis and oogenesis in vitro. These culture systems might be able to acquire a place in fertility testing, replacing in-vivo studies for ovarian function and female gamete quality testing. The proficiency data from a well-characterized follicle culture system suggest that this bioassay might be of potential use for in-vitro screening of xenobiotic substances affecting ovarian function and fertility.