Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets
Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets
复制标题
低剂量补充壳寡糖对断奶仔猪肠道形态、免疫反应、抗氧化能力和屏障功能的影响
DOI：
10.2527/jas.2014-7851
复制
发表时间：
2015-03-01
影响因子：
3.3
通讯作者：
Yin, Y. L.
中科院分区：
文献类型：
--
作者：
Xiong, X.;Yang, H. S.;Yin, Y. L.
本试验旨在研究饲粮中添加低剂量壳寡糖（COS）对断奶仔猪肠道形态、免疫反应、抗氧化能力和屏障功能的影响。试验选用120头21日龄断奶仔猪（平均体重7.86±0.22 kg）（6栏/日粮，10头/日粮），随机分为基础日粮（阴性对照）和基础日粮中添加COS （30 mg/kg）的2种饲粮处理，为期14 d。从每个处理中随机选择6头仔猪杀死进行血液和组织取样。治疗组与对照组的ADG、ADFI、G:F均无显著差异。断奶后第14 d，饲粮添加cos的仔猪胃pH值高于对照组（P < 0.05）。饲粮中添加COS可降低回肠绒毛高度（P < 0.05）和绒毛高度：隐窝深度（P < 0.05）。饲粮添加COS有降低十二指肠（P = 0.065）和空肠（P = 0.058）绒毛高度的趋势。治疗组对肠段隐窝深度无影响。饲粮中添加cos提高了仔猪十二指肠或空肠上皮内淋巴细胞数量和回肠杯状细胞数量（P < 0.05）。然而，COS显著降低了断奶仔猪回肠上皮内淋巴细胞数量（P < 0.05）。饲粮中添加cos的仔猪十二指肠、空肠和回肠IL-10和分泌性免疫球蛋白SIgA浓度均高于对照组（P < 0.05）。饲粮中添加COS可降低空肠和回肠总抗氧化能力和超氧化物歧化酶浓度（P < 0.05）。与对照组相比，饲粮中添加cos的仔猪回肠occludin、空肠和回肠ZO-1 mRNA表达量均有显著变化（P < 0.05）。综上所述，饲粮中添加30 mg/kg COS对断奶仔猪生长性能无促进作用，且有降低十二指肠或空肠绒毛高度的趋势。结果进一步表明，添加这一水平的COS可能会引起断奶仔猪小肠免疫和氧化应激反应，并破坏肠道屏障的完整性。本研究将为断奶仔猪低剂量添加COS提供指导。
This study was conducted to determine the effect of dietary supplementation of a low dose of chito-oligosaccharide (COS) on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets. A total of 120 weaned pigs (21 d of age; 7.86 +/- 0.22 kg average BW) were randomly assigned (6 pens/diet; 10 pigs/pen) to 2 dietary treatments consisting of a basal diet (negative control) or the basal diet supplemented with COS (30 mg/kg) for a 14-d period. Six randomly selected piglets from each treatment were killed for blood and tissue sampling. No significant differences were observed in ADG, ADFI, and G:F between treatment and the control group. Piglets fed the COS-supplemented diet had greater (P < 0.05) stomach pH than those fed the control diet on d 14 postweaning. Dietary supplementation with COS reduced villus height (P < 0.05) and villus height: crypt depth (P < 0.05) in the ileum. Dietary COS supplementation tended to reduce villus height in the duodenum (P = 0.065) and jejunum (P = 0.058). There was no effect on crypt depth in the intestinal segments of treatment group. Piglets fed the COS-supplemented diet increased (P < 0.05) the number of intraepithelial lymphocytes in duodenum or jejunum and goblet cells of ileum. However, COS decreased (P < 0.05) the number of intraepithelial lymphocytes in ileum of weaned piglets. The concentrations of IL-10 (duodenum, jejunum, and ileum) and secretory immunoglobulin (SIgA; duodenum and ileum) were higher in piglets fed the COS-supplemented diet compared with control (P < 0.05). Dietary COS supplementation reduced (P < 0.05) the concentration of total antioxidant capacity and superoxide dismutase of the jejunum or ileum. The mRNA expression of occludin in the ileum and ZO-1 in jejunum and ileum had a significant change in piglets fed the COS-supplemented diet compared with the control group (P < 0.05). In conclusion, these results indicated that dietary COS supplementation at 30 mg/kg had no effects on promoting growth performance and tended to reduce villus height in the duodenum or jejunum of weaned piglets. The results further showed that supplemental COS at this level may cause an immune and oxidative stress response in small intestine and have compromised the intestinal barrier integrity in weaned piglets. The research will provide guidance on the low dosage of COS supplementation on weaning pigs.