LIPID-PEROXIDATION MEASURED AS THIOBARBITURIC ACID-REACTIVE SUBSTANCES IN TISSUE-SLICES - CHARACTERIZATION AND COMPARISON WITH HOMOGENATES AND MICROSOMES
LIPID-PEROXIDATION MEASURED AS THIOBARBITURIC ACID-REACTIVE SUBSTANCES IN TISSUE-SLICES - CHARACTERIZATION AND COMPARISON WITH HOMOGENATES AND MICROSOMES
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DOI：
10.1016/0891-5849(88)90023-8
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发表时间：
1988-01-01
影响因子：
7.4
通讯作者：
TAPPEL, AL
中科院分区：
文献类型：
--
作者：
FRAGA, CG;LEIBOVITZ, BE;TAPPEL, AL
用肝切片检测溴化三氯甲烷、叔丁基氢过氧化氢(t-BOOH)和亚铁引起的脂质过氧化反应。将肝组织匀浆和微粒体对氧化条件的反应与组织切片的反应进行比较。通过硫代巴比妥酸反应物质(TBARS)的产生来评估脂质过氧化。正如在匀浆和微粒体中观察到的那样，肝切片产生的TBARS取决于组织的数量、孵育时间、诱导剂、诱导剂的数量和抗氧化剂的存在。对照组肝脏切片孵育温度37℃。C作用2 h，每克肝脏产生19 mmolTBARS。当切片在1 mM BrCCl3、1 mM t-BOOH或50微米亚铁的存在下孵育时，TBARS产量分别比控制值增加4.6倍、8.2倍或6.7倍。当这些制剂与相同的诱导剂孵育时，观察到肝匀浆和微粒体对TBARS的类似诱导。加入微米级的丁基羟基甲苯(BHT)，可阻止50微米亚铁诱导的肝组织损伤反应。结果表明，组织切片在测量脂质过氧化方面是有用的。在毒理学、药理学和营养学研究中，减少实验动物的数量是一个相关的问题，当研究了许多化合物或组织并且需要组织完整性时，强调组织切片的有用性。
Liver slices were used to measure lipid peroxidation induced by bromotrichlormethane, tert-butyl hydroperoxide (t-BOOH), or ferrous iron. The responses of liver homogenates and microsomes to oxidative conditions were compared with the response of tissue slices. Lipid peroxidation was evaluated by the production of thiobarbituric acid-reactive substances (TBARS). As was observed in homogenates and microsomes, TBARS production by liver slices depended upon the amount of tissue, the incubation time, inducer, the amount of inducer, and the presence of antioxidant. Control liver slices incubated at 37.degree. C for 2 h produced 19 mmol of TBARS per g of liver. When slices were incubated in the presence of 1 mM BrCCl3, 1 mM t-BOOH, or 50 .mu.M ferrous iron, TBARS production increased 4.6-, 8.2-, or 6,7-fold over the control value, respectively. Comparable induction of TBARS by liver homogenates and microsomes was observed when these preparations were incubated with the same inducers. Addition of .mu.M butylated hydroxytoluene (BHT) prevented the induction of TBARS by 50 .mu.M ferrous iron by liver slice. The results indicate the usefulness of tissue slices to measure lipid peroxidation. The usefulness of tissue slices is emphasized when a number of compounds or tissues are studied and tissue integrity is desired as in toxicological, pharmacological, and nutritional studies where reduced numbers of experimental animals is a relevant issue.