EXPRESSION OF GENE-RRG IS ASSOCIATED WITH REVERSION OF NIH 3T3 TRANSFORMED BY LTR-C-H-RAS
EXPRESSION OF GENE-RRG IS ASSOCIATED WITH REVERSION OF NIH 3T3 TRANSFORMED BY LTR-C-H-RAS
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DOI：
10.1126/science.1697103
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发表时间：
1990-08-17
期刊：
影响因子：
56.9
通讯作者：
FRIEDMAN, RM
中科院分区：
文献类型：
--
作者：
CONTENTE, S;KENYON, K;FRIEDMAN, RM
A partial complementary DNA was isolated for a gene (rrg) that is normally expressed in mouse NIH 3T3 cells, but is down-regulated after cellular transformation by long terminal repeat (LTR)-activated c-H-ras (LTR-c-H-ras). This gene was reexpressed in a nontumorigenic persistent revertant cell line created by prolonged treatment of the transformed cells with mouse interferon .alpha./.beta.. Persistent revertants stably transfected with rrg complementary DNA antisense expression vectors appeared transformed, had decreased amounts of rrg messenger RNA, and were tumorigenic in nude mice. Stable transfection with sense constructs did nto alter the normal morphology, message level, or nontumorigenicity of the persistent revertant cell line.
