Structure of the Plant Alternative Oxidase
Structure of the Plant Alternative Oxidase
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植物替代氧化酶的结构
DOI：
--
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发表时间：
2002
影响因子：
4.8
通讯作者：
A. Moore
中科院分区：
文献类型：
--
作者：
M. Albury;C. Affourtit;Paul G. Crichton;A. Moore
所有高等植物和许多真菌都含有一种替代氧化酶(AOX)，它从细胞色素途径的苯醌水平分支而来。首先，为了区分这种双铁蛋白的两种结构模型，其次，为了研究两个高度保守的酪氨酸残基的作用，我们在裂殖酵母中表达了一系列定点突变。线粒体呼吸分析显示，表达Glu-217或Glu-270突变的AOX蛋白的S.pombe细胞不再表现出抗霉素抗性的氧摄取，表明这些残基是AOX活性所必需的。尽管这些数据证实了将AOX描述为界面膜蛋白的模型，但它们与最近提出的其双铁中心的连接球体并不完全一致。我们进一步证明，当Tyr-253和Tyr-275突变为苯丙氨酸时，AOX活性分别完全保持或取消。这些数据是参考两个残基在AOX催化循环中的重要性来讨论的。
All higher plants and many fungi contain an alternative oxidase (AOX), which branches from the cytochrome pathway at the level of the quinone pool. In an attempt, first, to distinguish between two proposed structural models of this di-iron protein, and, second, to examine the roles of two highly conserved tyrosine residues, we have expressed an array of site-specific mutants inSchizosaccharomyces pombe. Mitochondrial respiratory analysis reveals that S. pombe cells expressing AOX proteins in which Glu-217 or Glu-270 were mutated, no longer exhibit antimycin-resistant oxygen uptake, indicating that these residues are essential for AOX activity. Although such data corroborate a model that describes the AOX as an interfacial membrane protein, they are not in full agreement with the most recently proposed ligation sphere of its di-iron center. We furthermore show that upon mutation of Tyr-253 and Tyr-275 to phenylalanines, AOX activity is fully maintained or abolished, respectively. These data are discussed in reference to the importance of both residues in the catalytic cycle of the AOX.
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影响因子：
56.9
作者：
Hoganson, CW;Babcock, GT
通讯作者：
Babcock, GT
影响因子：
56.9
作者：
Stowell, MHB;McPhillips, TM;Feher, G
通讯作者：
Feher, G
DOI：
10.1073/pnas.85.22.8487
发表时间：
1988-11-01
影响因子：
11.1
作者：
ALLEN, JP;FEHER, G;REES, DC
通讯作者：
REES, DC
影响因子：
56.9
作者：
Proshlyakov, DA;Pressler, MA;Babcock, GT
通讯作者：
Babcock, GT