Tritiated thymidine autoradiographic study on the origin and renewal of argentaffin cells in the pyloric gland of hamsters
Tritiated thymidine autoradiographic study on the origin and renewal of argentaffin cells in the pyloric gland of hamsters
复制标题
仓鼠幽门腺亲银细胞起源和更新的氚胸苷放射自显影研究
DOI：
10.1007/bf00222771
复制
发表时间：
1977
影响因子：
3.6
通讯作者：
S. Fujita
中科院分区：
文献类型：
--
作者：
T. Hattori;H. Niki;S. Fujita
采用3h -胸苷嘧啶闪光、累积和脉冲标记放射自显影技术研究了仓鼠幽门腺体中argentaffin细胞的起源和更新。用快速红B盐重氮法鉴定阿根廷蛋白细胞。闪光标记放射自显影显示，位于幽门黏膜中层至下层的argentaffin细胞未被3h -胸腺嘧啶标记，表明该细胞类型无增殖活性。在累积标记的第10天和第20天，腺体中分别有31%和63%的阿根廷蛋白细胞被标记。标记的阿根廷蛋白细胞集中在腺体上部（峡部周围），而在腺体最下层的细胞核上未发现标记。这些被标记的细胞显示在脉冲标记后的几天内向下迁移。它们被未标记的（弱或极弱标记的）细胞取代，这些细胞出现在峡部区域。幽门腺中的阿根廷蛋白细胞被认为起源于峡部的上皮前体细胞。经脉冲标记后，发现腺体中标记的阿根廷蛋白细胞数量呈指数型减少。这表明，argentaffin细胞的寿命不是固定的，但它们的更新符合“随机损失系统”。该细胞群的周转半衰期平均为15天。
The origin and renewal of the argentaffin cells in the pyloric glands of hamsters were studied by flash, cumulative and pulse labelling autoradiography with3H-thymidine. The argentaffin cells were identified by the Diazo Method using Fast Red B Salt.By flash labelling autoradiography, it was shown that the argentaffin cells located from the middle to the lower level of the pyloric mucosa were not labelled with3H-thymidine, indicating that this cell type has no proliferative activity. On the 10th and the 20th day of cumulative labelling, 31% and 63% of the argentaffin cells in the gland were found to be labelled, respectively. The labelled argentaffin cells were concentrated in the upper part of the gland (around the region of the isthmus), and no label was found over nuclei of the cells at the lowermost level of the gland. These labelled cells were shown to undergo a downward migration in the days following pulse labelling. They were replaced by unlabelled (and weakly or very weakly labelled) cells which arose at the region of the isthmus. The argentaffin cells in the pyloric gland are thought to arise from epithelial precursor cells at the region of the isthmus.The labelled argentaffin cells in the gland were found to decrease in number almost exponentially after pulse labelling. This indicates that the life span of argentaffin cells is not fixed, but their renewal conforms to the “random loss system”. The half time of turnover of this cell population was 15 days on average.