Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation.
Study of the effect of membrane thickness on microcapsule strength, permeability, and cell proliferation.
复制标题
DOI：
10.1002/jbm.a.34395
复制
发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Ying Ma;Ying Zhang;Yu Wang;Qiuyan Wang;M. Tan;Yang Liu;Li Chen;Nan Li;Weiting Yu;Xiao-jun Ma
中科院分区：
文献类型：
--
作者：
Ying Ma;Ying Zhang;Yu Wang;Qiuyan Wang;M. Tan;Yang Liu;Li Chen;Nan Li;Weiting Yu;Xiao-jun Ma
细胞微囊化是体外生产蛋白质或体内递送治疗产品的有前途的策略之一。膜厚度控制微胶囊的强度和渗透性，这可能反过来影响细胞生长和代谢。本研究考察了四组不同膜厚微胶囊的强度、渗透性以及包封后的中国仓鼠卵巢细胞的增殖和代谢情况。研究发现，增加膜厚度会增加微胶囊的强度，但会降低膜的渗透性。在前6天内，10μm厚膜微胶囊内的细胞增殖迅速，细胞密度可达到1.9×10(7)个细胞/mL微胶囊，细胞密度为92%。 15 μm膜厚度的微胶囊内细胞密度达到5.5×10(7)细胞/mL微胶囊，细胞密度>85%，并且这些微胶囊在11天后保持88%以上的完整率，远高于10 μm膜厚度的微胶囊。厚度超过20μm的膜由于蛋白质扩散率低而不适合封装细胞培养。这些结果表明细胞在最薄的膜厚度内短期存活。有一个特定的膜厚度更适合细胞长期培养的生长。这些发现将有助于根据各种目的制备具有所需膜厚度的微胶囊，用于微胶囊细胞培养。
Cell microencapsulation is one of the promising strategies for in vitro production of proteins or in vivo delivery of therapeutic products. Membrane thickness controls microcapsule strength and permeability, which may in return affect cell growth and metabolism. In this study, the strength, permeability, and encapsulated Chinese hamster ovary cell proliferation and metabolism of four groups of microcapsules with different membrane thicknesses were investigated. It was found that increasing membrane thickness increases microcapsule strength, whereas decreases membrane permeability. During the first 6 days, cells within microcapsules with 10 μm thickness membrane proliferated fast and could reach a cell density of 1.9 × 10(7) cells/mL microcapsule with 92% cell density. A cell density of 5.5 × 10(7) cells/mL microcapsule with >85% cell density was achieved within microcapsules with 15 μm membrane thickness and these microcapsules kept over 88% integrity ratio after 11 days, which was much higher than that of microcapsules with 10 μm membrane thickness. Membrane with more than 20 μm thickness was not suited for encapsulated cell culture owing to low-protein diffusion rate. These results indicated that cells survived shortly within the thinnest membrane thickness. There was a specific membrane thickness more suitable for cell growth for a long-time culture. These findings will be useful for preparing microcapsules with the desired membrane thickness for microencapsulated cell culture dependent on various purposes.