Correlation of cyclin D1 and Rb gene expression with apoptosis in invasive breast cancer
Correlation of cyclin D1 and Rb gene expression with apoptosis in invasive breast cancer
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DOI：
10.1136/mp.51.1.30
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发表时间：
1998-02-01
期刊：
影响因子：
--
通讯作者：
Baak, JPA
中科院分区：
文献类型：
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作者：
de Jong, JS;van Diest, PJ;Baak, JPA
体外研究表明，cyclin D1基因的扩增和过表达可以加速细胞通过G(1)期的进程。因此，cyclin D1可能具有抑制细胞凋亡的作用。视网膜母细胞瘤（Rb）基因最近被证明是细胞凋亡的重要调控因子。目的：探讨97例浸润性乳腺癌细胞中细胞周期蛋白D1和Rb基因的表达与凋亡计数的相关性。方法：石蜡包埋切片，免疫组化检测细胞周期蛋白D1和Rb基因的表达。在x630的放大倍数下，根据严格的方案，在10个视野范围内系统地分布在肿瘤最差分化的区域，计数凋亡细胞。凋亡细胞数以凋亡细胞/mm表示(2)。结果：49%的病例cyclin D1过表达。在44%的病例中发现Rb表达缺失，特别是在低分化肿瘤中。Cyclin D1与Rb表达呈正相关（p = 0.003）。凋亡计数从1到62/mm不等(2)。在总组和视网膜母细胞瘤蛋白（pRb）阳性肿瘤中，cyclin D1过表达与凋亡计数无显著相关性。Rb表达的缺失也与凋亡计数无关。结论：cyclin D1在pRb阳性肿瘤中经常过表达，但没有证据表明cyclin D1过表达或Rb表达在浸润性乳腺癌中具有抗凋亡作用。
Background-In vitro studies have shown that amplification and overexpression of the cyclin D1 gene can accelerate the progress of cells through the G(1) phase. Therefore, cyclin D1 may have an apoptosis inhibiting effect. The retinoblastoma (Rb) gene was shown recently to be an important regulator of apoptosis.Aims-To evaluate whether expression of the cyclin D1 and Rb genes correlated with apoptotic counts in a group of 97 invasive breast cancers.Methods-Expression of the cyclin D1 and Rb genes was detected by standard immunohistochemistry using paraffin wax embedded sections. Apoptotic cells were counted according to a strict protocol, in 10 fields of vision systematically spread over the most poorly differentiated area of the tumour, at a magnification of x630. Apoptotic cells counts were expressed as apoptotic cells/mm(2).Results-Cyclin D1 overexpression was found in 49% of cases. Loss of Rb expression was found in 44% of cases, and occurred particularly in poorly differentiated tumours. Cyclin D1 and Rb expression showed a positive correlation (p = 0.003). Apoptotic counts varied from 1 to 62/mm(2). There were no significant correlations between cyclin D1 overexpression and apoptotic counts in the total group or in the retinoblastoma protein (pRb) positive tumours. Loss of Rb expression also showed no correlation with apoptotic counts.Conclusions-Cyclin D1 is frequently overexpressed in pRb positive tumours, but no evidence has been found for an anti-apoptotic effect of cyclin D1 overexpression or Rb expression in invasive breast cancer.