Defective expression of hematopoietic cell protein tyrosine phosphatase (HCP) in lymphoid cells blocks Fas-mediated apoptosis.
Defective expression of hematopoietic cell protein tyrosine phosphatase (HCP) in lymphoid cells blocks Fas-mediated apoptosis.
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淋巴细胞中造血细胞蛋白酪氨酸磷酸酶 (HCP) 的缺陷表达会阻碍 Fas 介导的细胞凋亡。
DOI：
10.1016/1074-7613(95)90143-4
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发表时间：
1995
期刊：
影响因子：
32.4
通讯作者：
Mountz,JD
中科院分区：
文献类型：
--
作者：
Su,X;Zhou,T;Wang,Z;Yang,P;Jope,RS;Mountz,JD
Fas交联后CEM-6细胞发生蛋白酪氨酸脱磷酸化，而Fas交联后MOLT-4细胞则无此现象，酪氨酸磷酸酶抑制剂钒酸银可抑制CEM-6细胞的凋亡。去磷酸化的时间进程和水平与造血细胞蛋白酪氨酸磷酸酶（HCP）活性升高有关，但与其他两种酪氨酸磷酸酶活性无关。在11株人和小鼠Fas阳性淋巴细胞系中，HCP的表达水平与Fas凋亡功能相关。重组HCP在MOLT-4细胞系中的表达将该Fas凋亡抗性细胞系转化为Fas凋亡敏感性细胞系。抗小鼠Fas抗体处理后，HCP突变型meVmeV小鼠淋巴器官Fas表达增加，Fas介导的细胞凋亡功能降低。因此，HCP介导的蛋白质去磷酸化参与了淋巴细胞中Fas受体信号的传递。
Protein tyroslne dephosphorylation after Fas crosslinking occurred in Fas apoptosis-sensitive CEM-6 cells but not in Fas apoptosls-resistant MOLT-4 cells, and apoptosis in the CEM-6 cells could be inhibited by the protein tyroslne phosphatase inhibitor, psrvanadate. The time course and level of dephosphorylatlon were correlated with Increased hematopoietlc cell protein tyrosine phosphatase (HCP) activlty, but not with the activity of two other tyrosine phosphatases. The level of expression of HCP was correlated with Fas apoptosis function in eleven human and murine Faspositive lymphoid cell lines. Expression of recombinant HCP In the MOLT-4 cell line converted this Fas apoptosis-resistant cell line to Fas apoptosis sensitive. HCP-mutant meVmeV mice exhibited Increased expresslon of Fas but decreased Fas-mediated apop tosis function in lymphold organs after anti-mouse Fas antibody treatment In vivo. Thus, HCP-mediated protein dephosphorylatlon Is Involved In the delivery of the Fas apoptosls signal in lymphold cells.