Rolling Circle Translation of Circular RNA in Living Human Cells.
Rolling Circle Translation of Circular RNA in Living Human Cells.
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DOI：
10.1038/srep16435
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发表时间：
2015-11-10
影响因子：
4.6
通讯作者：
Abe H
中科院分区：
文献类型：
--
作者：
Abe N;Matsumoto K;Nishihara M;Nakano Y;Shibata A;Maruyama H;Shuto S;Matsuda A;Yoshida M;Ito Y;Abe H
我们最近报道，在无细胞大肠杆菌翻译系统中，环状RNA通过滚环扩增（RCA）机制被有效地翻译。最近的研究表明，由外显子序列组成的环状RNA在人类细胞中非常丰富。然而，这些环状RNA是否可以在细胞内翻译成蛋白质仍不清楚。在这项研究中，我们制备了具有无限开放阅读框架的环状RNA，并测试了它们在真核系统中的翻译。环状RNA在兔网织红细胞裂解物中被翻译成长蛋白，而不存在任何用于内部核糖体进入的特定元件、聚腺苷酸尾或帽结构。真核生物中的翻译系统可以接受更简单的RNA作为环化合成蛋白质的模板。在这里，我们证明了环状RNA在活的人类细胞中通过RCA机制有效地翻译以产生丰富的蛋白质产物。这些发现表明，人类细胞中存在的外显子环状RNA的翻译比以前认为的更可能。
We recently reported that circular RNA is efficiently translated by a rolling circle amplification (RCA) mechanism in a cell-free Escherichia coli translation system. Recent studies have shown that circular RNAs composed of exonic sequences are abundant in human cells. However, whether these circular RNAs can be translated into proteins within cells remains unclear. In this study, we prepared circular RNAs with an infinite open reading frame and tested their translation in eukaryotic systems. Circular RNAs were translated into long proteins in rabbit reticulocyte lysate in the absence of any particular element for internal ribosome entry, a poly-A tail, or a cap structure. The translation systems in eukaryote can accept much simpler RNA as a template for protein synthesis by cyclisation. Here, we demonstrated that the circular RNA is efficiently translated in living human cells to produce abundant protein product by RCA mechanism. These findings suggest that translation of exonic circular RNAs present in human cells is more probable than previously thought.