Timing of ubiquitin synthesis and conjugation into protein A24 during the HeLa cell cycle.
Timing of ubiquitin synthesis and conjugation into protein A24 during the HeLa cell cycle.
复制标题
HeLa 细胞周期中泛素合成和与蛋白 A24 结合的时间。
DOI：
10.1016/0006-291x(80)91608-3
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发表时间：
1980
影响因子：
3.1
通讯作者：
Busch,H
中科院分区：
文献类型：
--
作者：
Goldknopf,IL;Sudhakar,S;Rosenbaum,F;Busch,H
为了确定A24蛋白的形成与染色质复制的关系，我们将35 S蛋氨酸掺入泛素、A24蛋白、组蛋白4和高迁移率蛋白（HMG） 1和2与胸苷阻断同步的HeLa细胞的DNA合成和细胞分裂进行了比较。泛素的生物合成及其偶联成A24蛋白的过程是平行发生的，主要是期间事件，在G1期早期速率最高，在细胞分裂期间速率最低。A24蛋白与组蛋白4的标记率表明，泛素-组蛋白2A的结合率很高，超过了组蛋白合成所占的比例。这种额外的结合在G1期早期和晚期最高，其在细胞周期中的分布与HMG 1和HMG 2的生物合成相似。
To determine the relationship between formation of protein A24 and replication of chromatin, incorporation of 35 S methionine into ubiquitin, protein A24, histone 4 and high mobility group proteins (HMG) 1 and 2 were compared to DNA synthesis and cell division in HeLa cells synchronized by thymidine block. The biosynthesis of ubiquitin and its coupling into protein A24 occurred in parrallel and were largely interphase events rising to a maximum rate in early G1 phase and declining to a minimum during cell division. The ratio of labeling of protein A24 to histone 4 indicated that a high rate of ubiquitin-histone 2A conjugation occurred, in excess of that accounted for by histone synthesis. This additional conjugation was highest in early and late G1 phase and its distribution during the cell cycle was similar to the biosynthesis of HMG 1 and 2.