Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells.
Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells.
复制标题
DOI：
10.1083/jcb.147.1.185
复制
发表时间：
1999-10-04
期刊：
影响因子：
--
通讯作者：
Tsukita S
中科院分区：
文献类型：
--
作者：
Morita K;Sasaki H;Furuse M;Tsukita S
认为内皮细胞中的紧密连接（TJ）决定血管通透性。最近，紧密连接蛋白-1至-15被鉴定为TJ链的主要组分。其中，claudin-5（也称为跨膜蛋白缺失的腭心面综合征[TMVCF]）的表达无处不在，甚至在器官缺乏上皮组织，这表明可能参与这种claudin物种在内皮TJ。然后，我们获得了claudin-6特异性多克隆抗体和识别claudin-5/TMVCF和claudin-6的多克隆抗体。在脑和肺中，用这些多克隆抗体的免疫荧光显微镜显示，claudin-5/TMVCF仅集中在所有血管段的内皮细胞的细胞-细胞边界处，但不集中在上皮细胞的细胞-细胞边界处。免疫电镜显示claudin-5/TMVCF是TJ链的一个组成部分。相比之下，在肾脏中，claudin-5/TMVCF信号仅限于动脉的内皮细胞，但在静脉和毛细血管中检测不到。此外，在我们检查的所有其他组织中，在某些血管段的内皮细胞中特异性检测到claudin-5/TMVCF，但在上皮细胞中未检测到。此外，当将claudin-5/TMVCF cDNA引入小鼠L成纤维细胞时，重建的TJ链类似于体内内皮细胞中的TJ链，即，与面部相关的细胞外TJ。这些发现表明，claudin-5/TMVCF是TJ链的内皮细胞特异性组分。
Tight junctions (TJs) in endothelial cells are thought to determine vascular permeability. Recently, claudin-1 to -15 were identified as major components of TJ strands. Among these, claudin-5 (also called transmembrane protein deleted in velo-cardio-facial syndrome [TMVCF]) was expressed ubiquitously, even in organs lacking epithelial tissues, suggesting the possible involvement of this claudin species in endothelial TJs. We then obtained a claudin-6–specific polyclonal antibody and a polyclonal antibody that recognized both claudin-5/TMVCF and claudin-6. In the brain and lung, immunofluorescence microscopy with these polyclonal antibodies showed that claudin-5/TMVCF was exclusively concentrated at cell–cell borders of endothelial cells of all segments of blood vessels, but not at those of epithelial cells. Immunoreplica electron microscopy revealed that claudin-5/TMVCF was a component of TJ strands. In contrast, in the kidney, the claudin-5/TMVCF signal was restricted to endothelial cells of arteries, but was undetectable in those of veins and capillaries. In addition, in all other tissues we examined, claudin-5/TMVCF was specifically detected in endothelial cells of some segments of blood vessels, but not in epithelial cells. Furthermore, when claudin-5/TMVCF cDNA was introduced into mouse L fibroblasts, TJ strands were reconstituted that resembled those in endothelial cells in vivo, i.e., the extracellular face–associated TJs. These findings indicated that claudin-5/TMVCF is an endothelial cell–specific component of TJ strands.
登录
查看更多内容
单个基因产物Claudin -1或-2，在成纤维细胞中重构紧密结合的连接链和新兵。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Fujimoto, K;Tsukita, S
通讯作者：
Tsukita, S
通过表达突变型紧密连接膜蛋白，细胞旁通透性和跨上皮电阻的功能性分离以及顶端-基底外侧膜内扩散屏障的破坏。
影响因子：
7.8
作者：
Balda, M S;Whitney, J A;Flores, C;Gonzalez, S;Cereijido, M;Matter, K
通讯作者：
Matter, K
DOI：
10.1083/jcb.141.7.1539
发表时间：
1998-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Furuse M;Fujita K;Hiiragi T;Fujimoto K;Tsukita S
通讯作者：
Tsukita S
DOI：
10.1016/0165-3806(96)00117-4
发表时间：
1996-10-23
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Kniesel, U;Risau, W;Wolburg, H
通讯作者：
Wolburg, H
DOI：
10.1083/jcb.123.2.293
发表时间：
1993-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Balda MS;Gonzalez-Mariscal L;Matter K;Cereijido M;Anderson JM
通讯作者：
Anderson JM