Tunable assembly of amyloid-forming peptides into nanosheets as a retrovirus carrier
Tunable assembly of amyloid-forming peptides into nanosheets as a retrovirus carrier
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将淀粉样蛋白形成肽可调组装成纳米片作为逆转录病毒载体。
DOI：
10.1073/pnas.1416690112
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发表时间：
2015-03-10
影响因子：
11.1
通讯作者：
Liu, Cong
中科院分区：
文献类型：
--
作者：
Dai, Bin;Li, Dan;Liu, Cong
使用和改造淀粉样蛋白作为纳米材料是生物纳米技术的蓬勃发展趋势。在这里，我们展示了我们发现的淀粉样蛋白结构，称为“淀粉样蛋白样纳米片”，由阿尔茨海默病 Aβ 的关键淀粉样蛋白形成片段形成。结合多种生物物理和计算方法，我们提出了一种纳米片的结构模型，该模型是通过垂直于原纤维轴堆叠淀粉样原纤维棘而形成的。我们进一步使用纳米片进行实验室逆转录病毒转导增强，并通过电子显微镜直接观察纳米片表面病毒的存在。此外，根据我们的结构模型，我们设计了具有不同功能的纳米片形成肽，阐明了合理设计具有新颖结构和功能的淀粉样蛋白材料的潜力。
Using and engineering amyloid as nanomaterials are blossoming trends in bionanotechnology. Here, we show our discovery of an amyloid structure, termed "amyloid-like nanosheet," formed by a key amyloid-forming segment of Alzheimer's A beta. Combining multiple biophysical and computational approaches, we proposed a structural model for the nanosheet that is formed by stacking the amyloid fibril spines perpendicular to the fibril axis. We further used the nanosheet for laboratorial retroviral transduction enhancement and directly visualized the presence of virus on the nanosheet surface by electron microscopy. Furthermore, based on our structural model, we designed nanosheet-forming peptides with different functionalities, elucidating the potential of rational design for amyloid-based materials with novel architecture and function.