Analysis of Lymphocytes Deformation in Microchannel Flows using Compound Drop Model
Analysis of Lymphocytes Deformation in Microchannel Flows using Compound Drop Model
复制标题
使用复合液滴模型分析微通道流动中的淋巴细胞变形
DOI：
10.1088/1742-6596/530/1/012012
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Sugimoto and K. Nakabe
中科院分区：
文献类型：
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作者：
K. Tatsumi;K. Haizumi;K. Sugimoto and K. Nakabe
为了了解淋巴细胞的物理特性，建立一个能够预测淋巴细胞在流动中运动和变形的数值模型，采用复合液滴模型对淋巴细胞在微通道收缩区的流动进行了三维数值模拟.本模型考虑了细胞质的剪切稀化效应，并通过在细胞内施加另一滴来包括细胞核。并与用高速摄像机记录淋巴细胞变形的实验结果进行了比较。讨论了淋巴细胞变形度DI的时间依赖性，以及流速和细胞核位置对DI的影响。结果表明，传统的液滴模型适用于牛顿流体的性质的细胞质不能正确预测的变形收缩区的非线性效应变得重要。本模型，另一方面，表现出良好的协议与测量之一。此外，细胞核对淋巴细胞的形状有显著影响。刚性核抑制细胞的变形，其位置的轻微偏移对细胞前后侧的变形率有显著影响。
To understand the physical properties of the lymphocytes and develop a numerical model that can predict the motion and deformation of lymphocytes in flows, three-dimensional numerical simulation of the lymphocytes flowing through the contraction region of the microchannel was carried out employing the compound drop model in this study. The present model considers the shear-thinning effect of the cytoplasm, and includes the nucleus by applying another drop inside the cell. The results were compared with the experimental ones measured by recording the lymphocyte deformation using the high-speed camera. The time-dependent characteristics of the deformation degree DI of the lymphocyte, and the effects of the flow rate and the nucleus position on the DI were discussed. The results showed that the conventional drop model applying the Newtonian fluid properties to the cytoplasm cannot predict the deformation in the contraction region correctly where the nonlinear effects become important. The present model, on the other hand, showed a good agreement with the measured one. Further, the nucleus showed a significant effect on the shape of the lymphocyte. The rigid nucleus supressed the cell deformation and a slight deviation of its position affected the deformation rate of the leading and trailing sides markedly.