Homeostatic Plasticity Shapes Cell-Type-Specific Wiring in the Retina.
Homeostatic Plasticity Shapes Cell-Type-Specific Wiring in the Retina.
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DOI：
10.1016/j.neuron.2017.04.016
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发表时间：
2017-05-03
期刊：
影响因子：
16.2
通讯作者：
Kerschensteiner D
中科院分区：
文献类型：
--
作者：
Tien NW;Soto F;Kerschensteiner D
来自不同突触前伙伴的会聚输入塑造了突触后神经元的反应。发育中的突触后神经元是否独立地与每个突触前伙伴建立连接，或者平衡输入以获得特定的反应尚不清楚。视网膜神经节细胞（RGC）接收来自双极细胞类型的会聚输入，具有不同的对比度反应和时间调谐。在这里，使用光遗传学激活和药物遗传学沉默，我们发现6型双极细胞（B6）主导ONα-RGC的兴奋性输入。我们产生了小鼠，其中B6细胞被选择性地从发育回路中去除（B6-DTA）。在B6-DTA小鼠中，ONα-RGC以细胞类型特异性方式调节与其他双极细胞的连接。他们招募新的伙伴，增加与一些现有伙伴的突触，并保持来自其他伙伴的持续输入。膜片钳记录显示，解剖重新布线精确地保留了ONα-RGC的对比度和时间频率响应功能，表明稳态可塑性在发育中的视网膜中形成细胞类型特异性布线，以稳定发送到大脑的视觉信息。
Convergent input from different presynaptic partners shapes the responses of postsynaptic neurons. Whether developing postsynaptic neurons establish connections with each presynaptic partner independently, or balance inputs to attain specific responses is unclear. Retinal ganglion cells (RGCs) receive convergent input from bipolar cell types with different contrast responses and temporal tuning. Here, using optogenetic activation and pharmacogenetic silencing, we found that type 6 bipolar cells (B6) dominate excitatory input to ONα-RGCs. We generated mice in which B6 cells were selectively removed from developing circuits (B6-DTA). In B6-DTA mice, ONα-RGCs adjusted connectivity with other bipolar cells in a cell-type-specific manner. They recruited new partners, increased synapses with some existing partners, and maintained constant input from others. Patch clamp recordings revealed that anatomical rewiring precisely preserved contrast- and temporal frequency response functions of ONα-RGCs, indicating that homeostatic plasticity shapes cell-type-specific wiring in the developing retina to stabilize visual information sent to the brain.