A dinoflagellate producer of pinnatoxin G, isolated from sub-tropical Japanese waters
A dinoflagellate producer of pinnatoxin G, isolated from sub-tropical Japanese waters
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DOI：
10.1016/j.hal.2011.05.006
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发表时间：
2011-09-01
期刊：
影响因子：
6.6
通讯作者：
Selwood, Andrew I.
中科院分区：
文献类型：
--
作者：
Smith, Kirsty F.;Rhodes, Lesley L.;Selwood, Andrew I.
从采集于日本冲绳的表层沉积物样品中分离出一种非钙质孢囊，并从孢囊中培养出甲藻（甲藻纲，多甲藻目）。来自两个不同采样点的两个分离株（CAWD 188和CAWD 190），每个分离株均从单个孢囊培养，产生ca. 11.9和15 pg细胞（-1）的pinnatoxin G，分别通过液相色谱-质谱（LC-MS）测定。未检测到其他海葵毒素。运动细胞和包囊出现形态相同的pinnatoxins E和F生产者从新西兰沃茨和pinnatoxins E，F，和G生产者从澳大利亚沃茨分离。运动细胞和包囊细胞的平均长度分别为25 μ m × 21.3 μ m和29 μ m × 25.5 μ m。大亚基核糖体DNA序列数据的分析表明，两个良好的支持株与日本和澳大利亚的分离株之间略有差异。(C)2011 Elsevier B. V.保留所有权利。
A thecate dinoflagellate (Peridiniales, Dinophyceae) was cultured from individual non-calcareous cysts, isolated from surface sediment samples collected in Okinawa, Japan. Two isolates (CAWD188 and CAWD190) from two different sampling sites, each cultured from an individual cyst, produced ca. 11.9 and 15 pg cell(-1) pinnatoxin G, respectively, as determined by liquid chromatography-mass spectrometric (LC-MS). No other pinnatoxins were detected. The motile cells and cysts appeared morphologically identical to those of pinnatoxins E and F producers isolated from New Zealand waters and pinnatoxins E, F, and G producers isolated from Australian waters. Motile and cysts cells measured an average of 25 mu m long x 21.3 mu m wide and 29 mu m long x 25.5 mu m wide, respectively. Analysis of the large subunit ribosomal DNA sequence data showed two well supported strains with slight differences between the Japanese and the Australasian isolates. (C) 2011 Elsevier B.V. All rights reserved.