Formation of lipid reserves in fat body and eggs of the yellow fever mosquito, Aedes aegypti
Formation of lipid reserves in fat body and eggs of the yellow fever mosquito, Aedes aegypti
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DOI：
10.1016/s0022-1910(00)00158-x
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发表时间：
2001-06-01
影响因子：
2.2
通讯作者：
Ibrahim, MM
中科院分区：
文献类型：
--
作者：
Ziegler, R;Ibrahim, MM
我们检测了成年雌性埃及伊蚊的脂质积累。雌性的脂肪体中含有大约100杯的脂质。摄入糖水后，7天内血脂增加到300马克杯。在第五天的血餐后，喂食糖的雌性在两天内卵巢内积累了120-140马克的脂质。与此同时，脂肪体的脂质含量下降了100微克，表明脂质从脂肪体转移到卵母细胞。预先标记脂肪体脂质的实验支持这一结论。将标记转移到卵母细胞中：成熟卵母细胞中脂质比放射性为预标记脂肪体脂质比放射性的80%。血粉的成分也被用来合成卵母细胞脂。饥饿四天的肥胖雌性身体只剩下27杯脂质。当给这些雌性喂食血时，它们的卵母细胞成熟了，尽管卵母细胞的数量减少了，卵巢中含有的脂质只有第一次喂食糖水的雌性卵巢的一半。这些女性的脂质只略微增加到36微克。这表明雌性伊蚊。埃及伊蚊使用糖来合成脂质，但它们也可以使用血液成分来达到这个目的。(C) 2001爱思唯尔科学有限公司版权所有
We examined the accumulation of lipids in adult females of the mosquito, Aedes aegypti. Females emerged with about 100 mug lipid in the fat body. With access to sugar water lipids increased over seven days to 300 mug. After a blood meal on day five, sugar-fed females accumulated 120-140 mug of lipids in their ovaries within 2 days. At the same time the lipid content of the fat body decreased by 100 mug, indicating transfer of lipids from fat body to oocytes. Experiments in which fat body lipids were prelabelled support this conclusion. Label was transferred to oocytes: in mature oocytes the specific radioactivity of lipids was 80% of the specific radioactivity of prelabeled fat body lipids. Components of blood meals are also used to synthesize oocyte Lipids. Fat bodies of females starved for four days had only 27 mug of lipids left. When these females were given a blood meal, they matured oocytes, although the number of ooyctes was reduced and ovaries contained only half the amount of lipids found in ovaries of females which had first fed on sugar water. Fat body Lipids of these females had only slightly increased to 36 mug. This demonstrates that female Ae. aegypti use sugar to synthesize lipids, but they can also use components of blood for this purpose. (C) 2001 Elsevier Science Ltd, All rights reserved.