Differential global gene expression in red and white skeletal muscle
Differential global gene expression in red and white skeletal muscle
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DOI：
10.1152/ajpcell.2001.280.4.c763
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发表时间：
2001-04-01
影响因子：
5.5
通讯作者：
Booth, FW
中科院分区：
文献类型：
--
作者：
Campbell, WG;Gordon, SE;Booth, FW
骨骼肌纤维类型之间的基因表达差异长期以来一直吸引着科学家，但在大多数情况下，以前的实验一次只报告了一两个基因的差异。采用进化的整体mRNA表达分析技术来确定雌性ICR小鼠（30-35 g）的白色四头肌（白色肌）和红色比目鱼肌（混合红色肌）之间的约3,000个mRNA的潜在差异表达。微阵列分析确定了49条在白色和混合红色骨骼肌之间差异表达的mRNA序列，包括新发现的肌肉类型之间的差异表达。例如，目前的发现增加了9个转录因子/辅助调节因子和3个信号蛋白的已知差异表达mRNA的数量。白色和混合红色肌肉之间mRNA表达多样性的扩展知识表明，不同纤维类型的肌肉之间可能存在相当复杂的表型调节。
The differences in gene expression among the fiber types of skeletal muscle have long fascinated scientists, but for the most part, previous experiments have only reported differences of one or two genes at a time. The evolving technology of global mRNA expression analysis was employed to determine the potential differential expression of similar to3,000 mRNAs between the white quad (white muscle) and the red soleus muscle (mixed red muscle) of female ICR mice (30-35 g). Microarray analysis identified 49 mRNA sequences that were differentially expressed between white and mixed red skeletal muscle, including newly identified differential expressions between muscle types. For example, the current findings increase the number of known, differentially expressed mRNAs for transcription factors/coregulators by nine and signaling proteins by three. The expanding knowledge of the diversity of mRNA expression between white and mixed red muscle suggests that there could be quite a complex regulation of phenotype between muscles of different fiber types.