Exogenous ethylene increases the viability of cryopreserved Dendrobium protocorm-like bodies by regulating the hydrogen peroxide and its mediated oxidative stress
Exogenous ethylene increases the viability of cryopreserved Dendrobium protocorm-like bodies by regulating the hydrogen peroxide and its mediated oxidative stress
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DOI：
10.1007/s11240-020-01988-y
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发表时间：
2021-01
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影响因子：
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通讯作者：
Lingling Zhang;R. Ren;Xue-ru Jiang;Hao Zhou;Yan Liu
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作者：
Lingling Zhang;R. Ren;Xue-ru Jiang;Hao Zhou;Yan Liu
超低温保存是种质资源长期保存的有效方法，但经过液氮保存后，有些物种/品种的存活率下降甚至为零。以金钗石斛（Dendrobium nobile Lindl.）类原球茎（PLBs）为材料，在玻璃化-超低温保存的预培养阶段添加外源乙烯。以“滨名湖梦”原球茎为材料，研究乙烯对原球茎低温保存后细胞存活率、过氧化氢（H2 O2）水平及氧化应激指标的影响。结果表明，外源乙烯（乙烯利，ETH）的添加能显著提高石斛类原球茎玻璃化超低温保存后的内源ACC含量和细胞存活率，降低内源H2 O2含量，提高过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性。而外源乙烯抑制剂（硝酸钴，Co）的添加显著降低了玻璃化法超低温保存原球茎的内源ACC含量和细胞存活率，显著提高了内源H2 O2水平，并显著降低了CAT、APX活性和抗坏血酸（阿萨）含量。这些结果表明，外源乙烯可以通过调节H2 O2及其介导的氧化应激来提高石斛原球茎超低温保存后的存活率。此外，乙烯调节内源性H2 O2水平的方式可能与抗氧化剂AsA-谷胱甘肽（GSH）循环系统密切相关。
Cryopreservation, an effective method for long-term preservation of germplasm resources, but after liquid nitrogen (LN) preservation, the survival rate of some species/cultivars has decreased or even zero. Exogenous ethylene additives are added to in the preculture stage of vitrification-cryopreservation using the protocorm-like bodies (PLBs) ofDendrobium nobileLindl. ‘Hamana Lake Dream’ as materials, to study the effects of ethylene on cell survival rate, the level of hydrogen peroxide (H2O2) and oxidative stress indicators of the cryopreserved PLBs. The results showed that the addition of exogenous ethylene (ethephon, ETH) can significantly improve the endogenous 1-aminocyclopropane-1-carboxylic acid (ACC) content and cell survival rate ofDendrobiumPLBs after cryopreservation by vitrification, reduce the content of endogenous H2O2, and increase the activity of catalase (CAT) and ascorbic acid peroxidase (APX). While, the addition of exogenous ethylene inhibitor (cobalt nitrate, Co), the endogenous ACC content and the cell survival rate of PLBs after cryopreservation by vitrification were significantly reduced and the levels of endogenous H2O2was significantly increased, and the activity of CAT, APX and ascorbic acid (AsA) content were significantly reduced. These observations suggest that exogenous ethylene can improve the survival rate ofDendrobiumPLBs after cryopreservation by regulating the H2O2and its mediated oxidative stress. In addition, the way ethylene regula tes endogenous H2O2levels may be closely related to the AsA-glutathione (GSH) antioxidant circulation system.