Poly(glycerol sebacate)-modified polylactic acid scaffolds with improved hydrophilicity, mechanical strength and bioactivity for bone tissue regeneration
Poly(glycerol sebacate)-modified polylactic acid scaffolds with improved hydrophilicity, mechanical strength and bioactivity for bone tissue regeneration
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聚癸二酸甘油酯改性聚乳酸支架具有改善的亲水性、机械强度和生物活性，用于骨组织再生
DOI：
10.1039/c5ra13334c
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发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Liu, Changsheng
中科院分区：
文献类型：
--
作者：
Shi, Hengsong;Gan, Qi;Liu, Changsheng
聚乳酸具有优异的机械强度和体内生物相容性，在生物医学工程应用中得到了广泛的研究。但其固有的脆性、降解速度慢、亲水性差等缺点大大阻碍了其成功应用。本研究将可生物降解的交联型弹性体聚二十二酸甘油酯(PGS)应用于骨组织工程支架材料的改性。采用氯化钠颗粒浸出法制备了一种高度互联、大孔、三维的聚乳酸支架，通过预模制二元共混物(B.B)和均匀的PGS表面涂层(S.C)将PGS预聚体(Pre-PGS)引入到有或没有氧等离子体处理(O.P.和D.C)的PLA基支架上。在130℃固化后，所得的聚乳酸/聚乙二醇支架均表现出良好的相互连接的开孔结构。B.B和S.C均能有效地提高聚乳酸的亲水性、降解性、韧性和延展性，其中氧等离子体处理后的S.C.效果最好。在9：1和7：3的比例下，OP法制备的支架的断裂应变分别由纯聚乳酸的8%提高到13%和24%。进一步的研究表明，增强的亲水性和增加的表面粗糙度是氧基等离子体处理上述积极效果的主要贡献因素。此外，该复合支架具有良好的矿化性能、良好的细胞生物相容性、增强了对骨髓间充质干细胞(BMSCs)的细胞黏附和成骨分化作用。本研究结果表明，氧基等离子体预处理的PGS表面涂层是一种有效的材料改性策略，在骨组织再生配方中具有良好的应用前景。
Polylactic acid (PLA) has been extensively researched in biomedical engineering applications due to its superior mechanical strength and biocompatibility in vivo. But the inherent brittleness, slow degradability and inferior hydrophilicity greatly hamper its successful application. Here, a biodegradable crosslinked elastomer poly(glycerol sebacate) (PGS) was adapted to modify PLA scaffold for bone tissue engineering in this study. A highly interconnected and large porous, three-dimensional (3D) PLA-based scaffold was prepared by a NaCl particulate-leaching method and the PGS prepolymer (pre-PGS) was introduced either by pre-molding binary blend (B.B) or by surface coating (S.C) of a homogeneous PGS onto PLA-based scaffolds with and without oxygen plasma pretreatment (O.P and D.C). After curing at 130 degrees C, the resulting PLA/PGS scaffolds all exhibited well interconnected open-cell structures. The incorporation of PGS to PLA both by B.B and S.C could effectively improve the hydrophilicity, degradation, toughness and ductility, and the best efficacy was observed for the S.C with the oxygen plasma pretreatment. Specifically, at the ratio of PLA/PGS 9 : 1 and 7 : 3, the fracture strain of the PLA/PGS scaffolds by O.P were improved from 8% (pure PLA) to 13% and 24%, respectively. Further studies indicated that enhanced hydrophilicity and increased surface roughness were the main contributors to the above positive effect of oxygen-based plasma treatment. Additionally, these hybrid PLA/PGS scaffolds exhibited good mineralization, high cell biocompatibility, and enhanced cell adhesion and osteogenic differentiation for bone mesenchymal stem cells (BMSCs), especially for scaffolds by S.C. The present results suggest that the surface coating of PGS with oxygen-based plasma pretreatment is an effective strategy to modify the properties of PLA and the hybrid PLA/PGS scaffold represents a promising candidate in the formulation of bone tissue regeneration.