Membrane-bound alcohol dehydrogenase is essential for glyceric acid production in Acetobacter tropicalis.
Membrane-bound alcohol dehydrogenase is essential for glyceric acid production in Acetobacter tropicalis.
复制标题
膜结合醇脱氢酶对于热带醋杆菌的甘油酸生产至关重要。
DOI：
10.5650/jos.60.489
复制
发表时间：
2011
影响因子：
1.5
通讯作者：
K. Sakaki
中科院分区：
文献类型：
--
作者：
H. Habe;Shun Sato;T. Fukuoka;D. Kitamoto;T. Yakushi;K. Matsushita;K. Sakaki
热带醋杆菌NBRC 16470可以从甘油产生高度对映体纯的D-甘油酸（D-GA; > 99%对映体过量）。研究膜结合乙醇脱氢酶（mADH）是否参与了A. tropicalis中的mADH亚基I（adhA）基因，构建了adhA缺失的A. tropicalis（ΔadhA）。由于ΔadhA不产生GA，我们证实mADH是甘油转化为GA所必需的。我们还克隆并测序了对应于adhA和adhB的整个区域，其编码mADH亚基II。该序列与其他醋酸杆菌属的mADH亚基同源性较高（84- 86%）.
Acetobacter tropicalis NBRC16470 can produce highly enantiomerically pure D-glyceric acid (D-GA; >99 % enantiomeric excess) from glycerol. To investigate whether membrane-bound alcohol dehydrogenase (mADH) is involved in GA production in A. tropicalis, we amplified part of the gene encoding mADH subunit I (adhA) using polymerase chain reaction and constructed an adhA-disrupted mutant of A. tropicalis (ΔadhA). Because ΔadhA did not produce GA, we confirmed that mADH is essential for the conversion of glycerol to GA. We also cloned and sequenced the entire region corresponding to adhA and adhB, which encodes mADH subunit II. The sequences showed high identities (84-86 %) with the equivalent mADH subunits from other Acetobacter spp.
影响因子：
5
作者：
Kanchanarach, Watchara;Theeragool, Gunjana;Matsushita, Kazunobu
通讯作者：
Matsushita, Kazunobu
影响因子：
3.9
作者：
H. Toyama;F. Mathews;F. Mathews;O. Adachi;O. Adachi;K. Matsushita;K. Matsushita
通讯作者：
H. Toyama;F. Mathews;F. Mathews;O. Adachi;O. Adachi;K. Matsushita;K. Matsushita