Transmission and Persistence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus among Veterinarians and Their Household Members
Transmission and Persistence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus among Veterinarians and Their Household Members
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DOI：
10.1128/aem.02803-14
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发表时间：
2015-01-01
影响因子：
4.4
通讯作者：
Schouls, L. M.
中科院分区：
文献类型：
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作者：
Bosch, T.;Verkade, E.;Schouls, L. M.
在2003年首次分离出牲畜相关的耐甲氧西林金黄色葡萄球菌(LA-MRSA)后，这种MRSA变种迅速成为荷兰国家MRSA监测中从人类获得的主要MRSA。以前的研究表明，与其他MRSA谱系相比，LA-MRSA在人与人之间传播的情况很少发生。然而，这些报告根据流行病学和有限的分离株分子特征描述了LA-MRSA的传播，因此很难评估是否确实发生了传播。在这项研究中，我们使用全基因组图谱(WGMS)来识别LA-MRSA在人与人之间可能的传播。为此，我们使用了源自一项为期两年的前瞻性纵向队列研究的LA-MRSA分离株，在该研究中，家畜兽医及其家庭成员被反复采样以确定是否存在金黄色葡萄球菌。LA-MRSA菌株间存在较大程度的基因变异。然而，来自同一兽医的分离株的图谱之间的变异性非常有限，表明每个兽医持续携带或重新获得相同的LA-MRSA菌株。WGMS的比较显示，LA-MRSA的传播很可能发生在几乎每个兽医家庭中。然而，每个家庭似乎只有一种LA-MRSA菌株参与传播。这一结果证实了我们之前的发现，即LA-MRSA在基因上是多样化的。此外，本研究表明，LA-MRSA存在人与人之间的传播，并且LA-MRSA的携带可以持续存在，从而为这种高度耐药的病原体在社区中的传播带来了潜在的风险。
After the first isolation of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in 2003, this MRSA variant quickly became the predominant MRSA obtained from humans as part of the Dutch national MRSA surveillance. Previous studies have suggested that human-to-human transmission of LA-MRSA, compared to that of other MRSA lineages, rarely occurs. However, these reports describe the transmission of LA-MRSA based on epidemiology and limited molecular characterization of isolates, making it difficult to assess whether transmission actually occurred. In this study, we used whole-genome maps (WGMs) to identify possible transmission of LA-MRSA between humans. For this, we used LA-MRSA isolates originating from a 2-year prospective longitudinal cohort study in which livestock veterinarians and their household members were repeatedly sampled for the presence of S. aureus. A considerable degree of genotypic variation among LA-MRSA strains was observed. However, there was very limited variability between the maps of the isolates originating from the same veterinarian, indicating that each of the veterinarians persistently carried or had reacquired the same LA-MRSA strain. Comparison of WGMs revealed that LA-MRSA transmission had likely occurred within virtually every veterinarian household. Yet only a single LA-MRSA strain per household appeared to be involved in transmission. The results corroborate our previous finding that LA-MRSA is genetically diverse. Furthermore, this study shows that transmission of LA-MRSA between humans occurs and that carriage of LA-MRSA can be persistent, thus posing a potential risk for spread of this highly resistant pathogen in the community.