Differences of cell surface marker expression between bone marrow- and kidney-derived murine mesenchymal stromal cells and fibroblasts
Differences of cell surface marker expression between bone marrow- and kidney-derived murine mesenchymal stromal cells and fibroblasts
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DOI：
10.14715/cmb/2016.62.12.3
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发表时间：
2016-01-01
影响因子：
1.6
通讯作者：
Rohwedel, J.
中科院分区：
文献类型：
--
作者：
Cakiroglu, F.;Osbahr, J. W.;Rohwedel, J.
间充质基质细胞（MSC）是未分化的，具有再生特性的多能成体细胞。由于其分离和培养的简单性，它们与治疗方法特别相关。由于MSC显示出与成纤维细胞几乎相同的细胞表面标志物的表达模式，因此已经进行了许多尝试来解决MSC与成纤维细胞之间的相似性和差异性。在这项研究中，我们的目的是从骨髓（BM）和肾脏分离小鼠MSC的特点相比，成纤维细胞。通过塑料粘附从小鼠肾脏、BM和腹部皮肤分离细胞，随后通过分析其建立集落形成单位成纤维细胞（CFU-F）的能力、其形态、其增殖、端粒酶活性和细胞表面抗原的表达以及其分化能力来表征。来自3种小鼠组织的塑料贴壁细胞显示出相似的形态、增殖特征和CFU-F构建能力。然而，虽然骨髓和肾脏的MSC分化成脂肪，软骨和成骨方向，成纤维细胞不能有效地这样做。此外，在成纤维细胞群体中发现端粒酶表达较低的趋势。与成纤维细胞相比，来自肾脏和BM的造血细胞以显著更高的水平表达MSC特异性细胞表面标志物CD 105和Sca-1，并且以显著更低的水平表达CD 117，从而与成纤维细胞区分开。此外，我们发现某些CD标记物在BM衍生的细胞或成纤维细胞中以更高水平特异性表达。这项研究表明，从不同器官分离的小鼠MSC表达某些特定的标志物，这使得它们的区分。
Mesenchymal stromal cells (MSC) are undifferentiated, multipotent adult cells with regenerative properties. They are particularly relevant for therapeutic approaches due to the simplicity of their isolation and cultivation. Since MSC show an expression pattern of cell surface marker, which is almost identical to fibroblasts, many attempts have been made to address the similarities and differences between MSC and fibroblasts. In this study we aimed to isolate murine MSC from bone marrow (BM) and kidney to characterize them in comparison to fibroblasts. Cells were isolated from murine kidney, BM and abdominal skin by plastic adherence and subsequently characterized by analysing their capability to build colony-forming unit-fibroblasts (CFU-F), their morphology, their proliferation, expression of telomerase activity and cell surface antigens as well as their differentiation capacity. Plastic adherent cells from the 3 mouse tissues showed similar morphology, proliferation profiles and CFU-F building capacities. However, while MSC from BM and kidney differentiated into the adipogenic, chondrogenic and osteogenic direction, fibroblasts were not able to do so efficiently. In addition, a tendency for lower expression of telomerase was found in the fibroblast population. Proliferating cells from kidney and BM expressed the MSC-specific cell surface markers CD105 and Sca-1 on a significantly higher and CD117 on a significantly lower level compared to fibroblasts and were thereby distinguishable from fibroblasts. Furthermore, we found that certain CD markers were specifically expressed on a higher level, either in BM-derived cells or fibroblasts. This study demonstrates that murine MSC isolated from different organs express certain specific markers, which enable their discrimination.