Mapping QTL for chlorophyll fluorescence kinetics parameters at seedling stage as indicators of heat tolerance in wheat
Mapping QTL for chlorophyll fluorescence kinetics parameters at seedling stage as indicators of heat tolerance in wheat
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DOI：
10.1007/s10681-014-1283-1
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发表时间：
2015-03-01
期刊：
影响因子：
1.9
通讯作者：
Jing, Ruilian
中科院分区：
文献类型：
--
作者：
Azam, Farooq I.;Chang, Xiaoping;Jing, Ruilian
高温或热胁迫是影响小麦生产的最重要的非生物胁迫之一。叶绿素荧光动力学参数是了解各种非生物胁迫对植物生理过程和耐热性影响的最有效、最可靠的参数。本研究旨在通过对小麦进行不同时间的热胁迫处理，利用数量性状基因座分析技术，对小麦生长早期PCFK的控制基因组区域进行鉴定。以中国小麦品种寒选10号和鲁麦14号杂交获得的双单倍体群体为材料，在38 A ℃下处理2、4、6和8 h，测定了光系统II的初始荧光、最大荧光、可变荧光和最大量子效率（PCFK）。共检测到37个QTL，其中13个在常温25 ℃下检测到，24个在胁迫温度38 ℃下检测到。在1A、1B、2B、4A和7D染色体上检测到稳定或一致表达的初始荧光、最大荧光和可变荧光QTL。此外，还将24个QTL聚在1A、1B、2B、3B、3D、4A、5A和7D染色体上的9个簇中。这些QTL热点区域沿着与稳定的QTL，应在未来的定位研究更好地了解叶绿素荧光动力学参数的遗传基础。
High temperature or heat stress is one of the most important abiotic stresses that affect wheat production in almost every part of the world. Parameters of chlorophyll fluorescence kinetics (PCFKs) are the most powerful and reliable characters available to understand the impact of various abiotic stresses on plant physiological processes and heat tolerance. The present research was aimed to identify genomic regions controlling PCFKs at early growth stages of wheat through quantitative trait loci analysis by applying heat stress for different duration of time. A doubled haploid population derived from the cross of two Chinese wheat cultivars Hanxuan 10 and Lumai 14 was exposed to 38 A degrees C for 2, 4, 6 and 8 h of heat stress and PCFKs (initial fluorescence, maximum fluorescence, variable fluorescence and maximum quantum efficiency of photosystem II) were measured. A total of 37 QTLs were identified for the target traits, among which 13 were detected under normal temperature of 25 A degrees C and the remaining 24 under the stressful temperature of 38 A degrees C. Stable or consistently expressed QTLs for initial, maximum and variable fluorescence were detected on chromosomes 1A, 1B, 2B, 4A and 7D. In addition, 24 QTLs were clustered in 9 clusters on chromosomes 1A, 1B, 2B, 3B, 3D, 4A, 5A and 7D. These QTL hot spot regions along with stable QTLs should be targeted for better understanding the genetic basis of chlorophyll fluorescence kinetics parameters in future mapping studies.