Passive mechanical behavior of human neutrophils: effect of cytochalasin B.
Passive mechanical behavior of human neutrophils: effect of cytochalasin B.
复制标题
人类中性粒细胞的被动机械行为：细胞松弛素 B 的作用。
DOI：
10.1016/s0006-3495(94)81012-4
复制
发表时间：
1994
影响因子：
3.4
通讯作者：
Waugh,RE
中科院分区：
文献类型：
--
作者：
Tsai,MA;Frank,RS;Waugh,RE
肌动蛋白是真核细胞中普遍存在的蛋白质。它在细胞运动以及维持和控制细胞形状方面起着重要作用。在这篇文章中，我们打算解决的贡献肌动蛋白的被动力学性能的人中性粒细胞。作为评估这种贡献的框架，中性粒细胞被建模为一个简单的粘性液滴与恒定的皮质（“表面”）张力。用细胞松弛素B（CT B）破坏F-肌动蛋白结构，用单细胞微管吸吮法测定中性粒细胞皮质张力和胞浆粘度。通过简单的力平衡计算皮质张力，并根据细胞进入微量移液管的数值分析计算粘度。CTB以剂量依赖性方式降低细胞皮质张力：在3 μ M浓度下降低19%，在30 μ M浓度下降低49%。当在相同的抽吸压力下比较时，CTB还在3 μ M的浓度下将细胞质粘度降低约-25%，并且在30 μ M的浓度下降低约65%。所有三组中性粒细胞、正常细胞和用3或30 μ M CTB处理的细胞均表现出非牛顿行为，因为表观粘度随着剪切速率的增加而降低。细胞质粘度对变形速率的依赖性可以经验地描述为mu=mu c（γ m/γ c）-B，其中mu是细胞质粘度，γ m是平均剪切速率，mu c是在特征剪切速率γ c下的特征粘度，并且B是材料系数。（250字处删节）
Actin is a ubiquitous protein in eukaryotic cells. It plays a major role in cell motility and in the maintenance and control of cell shape. In this article, we intend to address the contribution of actin to the passive mechanical properties of human neutrophils. As a framework for assessing this contribution, the neutrophil is modeled as a simple viscous fluid drop with a constant cortical ("surface") tension. The reagent cytochalasin B (CTB) was used to disrupt the F-actin structure, and the neutrophil cortical tension and cytoplasmic viscosity were evaluated by single-cell micropipette aspiration. The cortical tension was calculated by simple force balance, and the viscosity was calculated according to a numerical analysis of the cell entry into the micropipette. CTB reduced the cell cortical tension in a dose-dependent fashion: by 19% at a concentration of 3 microM and by 49% at 30 microM. CTB also reduced the cytoplasmic viscosity by approximately -25% at a concentration of 3 microM and by approximately 65% at a concentration of 30 microM when compared at the same aspiration pressures. All three groups of neutrophils, normal cells, and cells treated with either 3 or 30 microM CTB, exhibited non-Newtonian behavior, in that the apparent viscosity decreased with increasing shear rate. The dependence of the cytoplasmic viscosity on deformation rate can be described empirically by mu=mu c(gamma m/gamma c)-b, where mu is cytoplasmic viscosity, gamma m is mean shear rate, mu c is the characteristic viscosity at the characteristic shear rate gamma c, and b is a material coefficient.(ABSTRACT TRUNCATED AT 250 WORDS)