Intestinal epithelial cells both express and respond to interleukin 15
Intestinal epithelial cells both express and respond to interleukin 15
复制标题
DOI：
10.1016/s0016-5085(96)70036-7
复制
发表时间：
1996-12-01
期刊：
影响因子：
29.4
通讯作者：
Podolsky, DK
中科院分区：
文献类型：
--
作者：
Reinecker, HC;MacDermott, RP;Podolsky, DK
背景与目的：白细胞介素（Interleukin， IL）-15在淋巴细胞中发挥类似于IL-2的功能作用，IL-15在非淋巴细胞中表达，并可能整合这些细胞参与经典的免疫应答。本研究的目的是表征IL-15在肠上皮细胞中的表达并确定IL-15在粘膜免疫系统中的功能作用。方法：从大鼠空肠文库中克隆大鼠IL-15，采用Northern和Western blotting法检测IL-15在大鼠和人肠上皮细胞中的表达，采用免疫沉淀法检测肠上皮细胞中酪氨酸激酶对IL-15的激活。大鼠和人肠上皮细胞表达IL-15信使RNA， IL-15激活Stat3，刺激肠上皮细胞增殖，IL-15在人原代肠上皮细胞中转录物的证实，支持了这一观察结果与体内肠上皮细胞功能的相关性。结论：IL-15可通过肠上皮细胞表达，并可能调节肠上皮细胞功能，提示IL-15是肠上皮细胞功能与肠道免疫系统整合的重要介质。
Background & Aims: Interleukin (IL)-15 exerts functional effects on lymphocytes similar to those of IL-2, IL-15 is expressed by nonlymphoid cells and may integrate these cells into classical immune responses, The aim of this study was to characterize the expression of IL-15 by intestinal epithelial cells and determine the functional roles of IL-15 within the mucosal immune system. Methods: Rat IL-15 was cloned from a rat jejunal library, Expression of IL-15 in rat and human intestinal epithelial cells was assessed by Northern and Western blotting, Tyrosine kinase activation in response to IL-15 in intestinal epithelial cells was determined by immunoprecipitation, Results: Rat and human intestinal epithelial cells express IL-15 messenger RNA, IL-15 activates Stat3 and stimulates the proliferation of intestinal epithelial cells, The relevance of the observations for intestinal epithelial cell function in vivo was supported by the demonstration of transcripts for IL-15 in primary human intestinal epithelial cells. Conclusions: IL-15 is expressed by intestinal epithelial cells and may be able to regulate intestinal epithelial cell function, These experiments suggest that IL-15 is an important mediator that could integrate intestinal epithelial cell function with the intestinal immune system.