A serological assay to detect SARS-CoV-2 seroconversion in humans
A serological assay to detect SARS-CoV-2 seroconversion in humans
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DOI：
10.1101/2020.03.17.20037713
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发表时间：
2020-05-12
期刊：
影响因子：
82.9
通讯作者：
Krammer, Florian
中科院分区：
文献类型：
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作者：
Amanat, Fatima;Stadlbauer, Daniel;Krammer, Florian
建立一种检测人血清和血浆中SARS-CoV-2刺突蛋白抗体的酶联免疫吸附试验，在此，我们描述了一种用于筛选和鉴定人SARS-CoV-2血清转换者的血清学酶联免疫吸附试验。该检测试剂盒不需要处理感染性病毒，可进行调整以检测血清和血浆中的不同抗体类型，并可进行缩放。血清学检测对于帮助确定人群中SARS-CoV-2的既往暴露、识别用于恢复期血浆治疗的高反应性人类供体以及研究保护的相关性至关重要。
Development of an enzyme-linked immunosorbent assay to detect antibodies to the SARS-CoV-2 spike protein in human sera and plasma.Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection.