Programmed electrical stimulation in mice.
Programmed electrical stimulation in mice.
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DOI：
10.3791/1730
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发表时间：
2010-05-26
期刊：
影响因子：
--
通讯作者：
Wehrens, Xander H T
中科院分区：
文献类型：
--
作者：
Li, Na;Wehrens, Xander H T
转基因小鼠已成为研究传导异常、房性和室性心律失常以及心源性猝死的分子机制的较佳动物模型。(1)小鼠的心内起搏研究可以使用插入颈静脉的1.1F八管导管，并进入右心房和右心室。在这里，我们说明了在小鼠身上进行程序性电刺激所涉及的步骤。在心房、房室交界处和心肌同时记录体表心电图和心内电信号，而心内起搏是使用外部刺激器进行的。因此，小鼠的程序性电刺激为探索传导缺陷和心律失常的分子机制提供了独特的机会。
Genetically-modified mice have emerged as a preferable animal model to study the molecular mechanisms underlying conduction abnormalities, atrial and ventricular arrhythmias, and sudden cardiac death.(1) Intracardiac pacing studies can be performed in mice using a 1.1F octapolar catheter inserted into the jugular vein, and advanced into the right atrium and ventricle. Here, we illustrate the steps involved in performing programmed electrical stimulation in mice. Surface ECG and intracardiac electrograms are recorded simultaneously in the atria, atrioventricular junction, and ventricular myocardium, whereas intracardiac pacing of the atrium is performed using an external stimulator. Thus, programmed electrical stimulation in mice provides unique opportunities to explore molecular mechanisms underlying conduction defects and cardiac arrhythmias.