A model to predict the frequency of integration of fitness-related QTLs from cultivated to wild soybean
A model to predict the frequency of integration of fitness-related QTLs from cultivated to wild soybean
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DOI：
10.1007/s11248-011-9516-8
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发表时间：
2012-02-01
影响因子：
3
通讯作者：
Ohsawa, R.
中科院分区：
文献类型：
--
作者：
Kitamoto, N.;Kaga, A.;Ohsawa, R.
随着转基因产品的扩散和转基因作物土地利用的指数增长，越来越需要开发定量方法来评估转基因通过自然杂交逃逸到作物近缘野生种群中的风险。本研究以野生大豆G.大豆×栽培大豆模拟开始于10个F-1和990个野生大豆通过自交或异交繁殖。种子产量由两个种子数（SN）QTL的遗传效应决定。每粒种子根据在3个冬季存活（WS）QTL的母体基因型越冬。我们假设一个中性转基因插入在不同的位点，并计算其灭绝率。G. SN和WS QTL的max等位基因显著降低了中性转基因在所有插入位点的渐渗概率。G. WS QTL上的max等位基因比SN QTL上的max等位基因更能降低风险。虽然大多数模型研究只集中在转基因的基因型效应，我们表明，健身相关的驯化基因的存在下，转基因逃逸的风险有很大的影响。我们的模型提供了考虑驯化基因和转基因的影响，他们可以广泛应用于其他野生型×作物相对复杂的优势。
With the proliferation of genetically modified (GM) products and the almost exponential growth of land use for GM crops, there is a growing need to develop quantitative approaches to estimating the risk of escape of transgenes into wild populations of crop relatives by natural hybridization. We assessed the risk of transgene escape by constructing a population genetic model based on information on fitness-related QTLs obtained from an F-2 population of wild soybean G. soja x cultivated soybean Glycine max. Simulation started with ten F-1 and 990 wild soybeans reproducing by selfing or out-crossing. Seed production was determined from the genetic effects of two QTLs for number of seeds (SN). Each seed survived winter according to the maternal genotype at three QTLs for winter survival (WS). We assumed that one neutral transgene was inserted at various sites and calculated its extinction rate. The presence of G. max alleles at SN and WS QTLs significantly decreased the probability of introgression of the neutral transgene at all insertion sites equally. The presence of G. max alleles at WS QTLs lowered the risk more than their presence at SN QTLs. Although most model studies have concentrated only on genotypic effects of transgenes, we show that the presence of fitness-related domestication genes has a large effect on the risk of transgene escape. Our model offers the advantage of considering the effects of both domestication genes and a transgene, and they can be widely applied to other wild x crop relative complexes.