A possible role of Drosophila CTCF in mitotic bookmarking and maintaining chromatin domains during the cell cycle.
A possible role of Drosophila CTCF in mitotic bookmarking and maintaining chromatin domains during the cell cycle.
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果蝇 CTCF 在细胞周期中有丝分裂书签和维持染色质结构域中的可能作用
DOI：
10.1186/s40659-015-0019-6
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发表时间：
2015-05-27
影响因子：
6.7
通讯作者：
Zhao Z
中科院分区：
文献类型：
--
作者：
Shen W;Wang D;Ye B;Shi M;Zhang Y;Zhao Z
背景：CCCTC结合因子(CTCF)是一种高度保守的绝缘蛋白，在许多细胞过程中发挥着不同的作用。CTCF是高等真核生物中的主要结构蛋白之一，与其他结构蛋白和调控因子结合，也塑造了基因组的三维结构。实验表明，CTCF在有丝分裂过程中仍与染色质部分相关。然而，CTCF在整个细胞周期中维持和繁殖基因组结构中的作用仍不清楚。结果：我们对果蝇CTCF(DCTCF)的公共数据集进行了全面的生物信息学分析。我们根据dCTCF结合位点在细胞周期中的占据状态对其进行了表征，并将其分为三类：间期-有丝分裂-普通(IM)、仅间期(IO)和仅有丝分裂(MO)。综合功能分析表明，不同类别的dCTCF结合位点可能参与不同的生物学过程，IM结合位点更保守，结合更紧密。结论：dCTCF在细胞周期中具有不同的功能，提示dCTCF可能通过在整个细胞周期中维持局部染色质区段而有助于果蝇基因组三维结构的建立。
Background:The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensional organization of a genome. Experiments show CTCF partially remains associated with chromatin during mitosis. However, the role of CTCF in the maintenance and propagation of genome architectures throughout the cell cycle remains elusive.Results:We performed a comprehensive bioinformatics analysis on public datasets of Drosophila CTCF (dCTCF). We characterized dCTCF-binding sites according to their occupancy status during the cell cycle, and identified three classes: interphase-mitosis-common (IM), interphase-only (IO) and mitosis-only (MO) sites. Integrated function analysis showed dCTCF-binding sites of different classes might be involved in different biological processes, and IM sites were more conserved and more intensely bound. dCTCF-binding sites of the same class preferentially localized closer to each other, and were highly enriched at chromatin syntenic and topologically associating domains boundaries.Conclusions:Our results revealed different functions of dCTCF during the cell cycle and suggested that dCTCF might contribute to the establishment of the three-dimensional architecture of the Drosophila genome by maintaining local chromatin compartments throughout the whole cell cycle.
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影响因子：
7
作者：
Kellner WA;Ramos E;Van Bortle K;Takenaka N;Corces VG
通讯作者：
Corces VG
影响因子：
11.4
作者：
Heath, Helen;de Almeida, Claudia Ribeiro;Galjart, Niels
通讯作者：
Galjart, Niels
影响因子：
16.8
作者：
Nekrasov, Maxim;Amrichova, Jana;Tremethick, David J.
通讯作者：
Tremethick, David J.
DOI：
10.1126/science.1236083
发表时间：
2013-11-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Naumova N;Imakaev M;Fudenberg G;Zhan Y;Lajoie BR;Mirny LA;Dekker J
通讯作者：
Dekker J
影响因子：
14.9
作者：
Jothi, Raja;Cuddapah, Suresh;Barski, Artem;Cui, Kairong;Zhao, Keji
通讯作者：
Zhao, Keji