Development of PLGA-PEG-COOH and Gelatin-Based Microparticles Dual Delivery System and E-Beam Sterilization Effects for Controlled Release of BMP-2 and IGF-1
Development of PLGA-PEG-COOH and Gelatin-Based Microparticles Dual Delivery System and E-Beam Sterilization Effects for Controlled Release of BMP-2 and IGF-1
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DOI：
10.1002/ppsc.202000180
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发表时间：
2020-08-18
影响因子：
2.7
通讯作者：
Yang, Yunzhi Peter
中科院分区：
文献类型：
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作者：
Bai, Yan;Moeinzadeh, Seyedsina;Yang, Yunzhi Peter
研制了一种以聚乳酸-羟基乙酸共聚物-聚乙二醇-羧基（PLGA-PEG-COOH）和明胶为基质的微球（MPs）双重释放系统，用于释放骨形态发生蛋白-2（BMP-2）和胰岛素样生长因子-1（IGF-1）。递送系统基于其形态学、负载能力、包封效率和释放动力学进行表征。研究了电子束灭菌对BMP-2和IGF-1负载的MP的影响及其生物学效应。评价了BMP-2和IGF-1的受控双重释放对人间充质干细胞（MSC）成骨的协同作用。生长因子在明胶和PLGA-PEG-COOH MP中的包封效率在64.78%至76.11%的范围内。电子束灭菌的生长因子递送系统在显著促进MSC的成骨方面是有效的，尽管电子束灭菌使MP中的生长因子的生物活性降低了约22%。BMP-2从明胶MP/PEG水凝胶的释放行为显示出比IGF-1从PLGA-PEG-COOH MP/PEG水凝胶的释放（27.3%）更快的释放（52.7%）。结果表明，基于明胶和PLGA-PEG-COOH MP的递送系统可以通过将不同的生长因子掺入不同的微粒中来实现治疗性生物分子的暂时释放。电子束灭菌是一种简便易行的生长因子载体灭菌方法，可为生长因子载体的临床应用铺平道路。
A poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) and gelatin-based microparticles (MPs) dual delivery system for release of bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP-2 and IGF-1-loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP-2 and IGF-1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA-PEG-COOH MPs is in the range of 64.78% to 76.11%. E-beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E-beam sterilization decreases the bioactivity of growth factors in MPs by approximate to 22%. BMP-2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF-1 from the PLGA-PEG-COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA-PEG-COOH MPs-based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors-loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.