Electron injection from mitochondrial transcription factor A to DNA associated with thymine dimer photo repair
Electron injection from mitochondrial transcription factor A to DNA associated with thymine dimer photo repair
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DOI：
10.1016/j.bmc.2018.11.044
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发表时间：
2019-01-15
影响因子：
3.5
通讯作者：
Sugiyama, Hiroshi
中科院分区：
文献类型：
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作者：
Hashiya, Fumitaka;Ito, Shinji;Sugiyama, Hiroshi
电子传递通过双链DNA的pi堆叠阵列有助于碱基的氧化还原化学，包括鸟嘌呤氧化和胸腺嘧啶-胸腺嘧啶二聚体的光解酶修复。5-溴嘧啶是一种有吸引力的光反应胸腺嘧啶类似物，可用于研究DNA中的电子转移，是蛋白质-DNA相互作用分析的有用探针。在本研究中，我们发现紫外线照射促进了线粒体转录因子A的电子注入DNA。我们还观察到这种电子注射可以导致胸腺嘧啶-胸腺嘧啶二聚体的修复。
Electron transfer through pi-stacked arrays of double-stranded DNA contributes to the redox chemistry of bases, including guanine oxidation and thymine-thymine dimer repair by photolyase. 5-Bromouracil is an attractive photoreactive thymine analogue that can be used to investigate electron transfer in DNA, and is a useful probe for protein-DNA interaction analysis. In the present study using U-Br we found that UV irradiation facilitated electron injection from mitochondrial transcription factor A into DNA. We also observed that this electron injection could lead to repair of a thymine-thymine dimer.