BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects
BMP-9-transduced prefabricated muscular flaps for the treatment of bony defects
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DOI：
10.1097/00001665-200409000-00007
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发表时间：
2004-09-01
影响因子：
0.9
通讯作者：
Ogle, RC
中科院分区：
文献类型：
--
作者：
Abdelaal, MM;Tholpady, SS;Ogle, RC
自体骨移植技术涉及使用需要从健康部位提取的组织。这可能导致供区严重发病，导致单部位缺损变为双部位缺损。骨移植物可能特别难以操作，因为骨是相对不可塑形的组织。此外，骨移植物不能容纳可移植的血管供应也限制了这种骨移植物的可能尺寸。由于这些局限性，一种具有血管供应的可塑形移植物在重建应用中具有显著的优势。在这项研究中，基因治疗技术被用来创造这样的移植物。将表达BMP-9的腺病毒注射到裸动物的背阔肌中以引起该肌肉的骨分化。通过逆转录聚合酶链反应和免疫组化检测骨中肌肉向软骨的分化，以确定皮瓣抬高的最佳时间。注射BMP-9病毒后，在3周内每周对动物进行活检。在研究期间，在这些组织中发现了骨和软骨标记物。最佳皮瓣抬高时间确定为注射病毒后2周。
Autologous bone grafting techniques involve the use of tissues that need to be extracted from healthy sites. This can lead to significant donor site morbidity that causes a one-site defect to become a two-site defect. Bone grafts can be especially difficult to manipulate, because bone is a relatively nonmoldable tissue. Furthermore, the inability of a bone graft to contain a transplantable vascular supply also limits the possible size that such a bone graft can be. Because of these limitations, a graft that was moldable with a vascular supply would possess significant advantages in reconstructive applications. In this research, gene therapy techniques were used to create such a graft. An adenovirus expressing BMP-9 was injected into the latissimus dorsi of a nude animal to cause bony differentiation of that muscle. Differentiation of the muscle to cartilage in bone was measured by reverse transcription polymerase chain reaction and immunohistochemistry to determine the optimal time of flap elevation. After injection of the BMP-9 virus, the animals were biopsied weekly over a 3-week period. Both bone and cartilage markers were discovered in these tissues over the study period. Optimal flap elevation time was established to be 2 weeks after injection of the virus.