Methylation of the estrogen receptor gene is associated with aging and atherosclerosis in the cardiovascular system
Methylation of the estrogen receptor gene is associated with aging and atherosclerosis in the cardiovascular system
复制标题
DOI：
10.1016/s0008-6363(99)00153-4
复制
发表时间：
1999-09-01
影响因子：
10.8
通讯作者：
Issa, JPJ
中科院分区：
文献类型：
--
作者：
Post, WS;Goldschmidt-Clermont, PJ;Issa, JPJ
目的：雌激素受体基因 α (ER α) 启动子区域的甲基化随着人类结肠年龄的变化而发生，并导致基因转录失活。在这项研究中，我们试图确定这种与年龄相关的甲基化是否发生在心血管系统中，以及它是否与动脉粥样硬化疾病有关。方法：我们采用Southern blot分析测定人光心房、主动脉、乳内动脉、隐静脉、冠状动脉粥样斑块切除样本以及培养的主动脉内皮细胞和平滑肌细胞中ERα基因的甲基化状态。结果：右心房 ER α 基因甲基化与年龄相关增加（范围 6% 至 19%，R = 0.36，P
Objective: Methylation of the promoter region of the estrogen receptor gene alpha (ER alpha) occurs as a function of age in human colon, and results in inactivation of gene transcription. In this study, we sought to determine whether such age-related methylation occurs in the cardiovascular system, and whether it is associated with atherosclerotic disease. Methods: We used Southern blot analysis to determine the methylation state of the ER alpha gene in human light atrium, aorta, internal mammary artery, saphenous vein, coronary atherectomy samples, as well as cultured aortic endothelial cells and smooth muscle cells. Results: An age related increase in ER alpha gene methylation occurs in the right atrium (range 6 to 19%, R = 0.36, P