Native mass spectrometry as a tool in structural biology.
Native mass spectrometry as a tool in structural biology.
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DOI：
10.1002/0471140864.ps1712s62
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发表时间：
2010-11-01
影响因子：
--
通讯作者：
van Duijn, Esther
中科院分区：
文献类型：
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作者：
Lorenzen, Kristina;van Duijn, Esther
天然质谱（native MS）提供有关蛋白质复合物的组成，拓扑排列，动力学和结构特性的信息。质量范围基本上是无限的和高度动态的，允许在同一测量内检测小亚基和大复合物。与大多数其他结构生物学方法相比，分析所需的蛋白质量非常低。本单元介绍了天然MS。它首先解释了如何测量完整蛋白质和蛋白质复合物的基本方法和细节，并继续研究气相中的动力学和复合物稳定性。最后一节讨论了最近的扩展到本地MS领域，离子迁移率，它允许直接评估感兴趣的复合物的结构特性。
Native mass spectrometry (native MS) gives information about the composition, topological arrangements, dynamics, and structural properties of protein complexes. The mass range is principally unlimited and highly dynamic, allowing the detection of small subunits and large complexes within the same measurement. The amount of protein needed for an analysis is, compared to most other structural biology methods, very low. This unit provides an introduction to native MS. It starts with an explanation of the basic method and details on how to measure intact proteins and protein complexes, and continues with the study of dynamics and complex stability in the gas phase. The final section discusses the most recent extension to the native MS field, ion mobility, which allows the direct assessment of the structural properties of the complexes of interest.