Interspecies killing activity of Pseudomonas syringae tailocins
Interspecies killing activity of Pseudomonas syringae tailocins
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DOI：
10.1099/mic.0.001258
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发表时间：
2022-11-01
期刊：
影响因子：
2.8
通讯作者：
Baltrus，David A.
中科院分区：
文献类型：
--
作者：
Weaver，Savannah L.;Zhu，Libin;Baltrus，David A.
尾素是核糖体合成的细菌素，由细菌基因组编码，但最初来源于噬菌体尾部。与细菌素和噬菌体一样，tailocins在很大程度上被认为是物种特异性的，其杀伤活性通常被认为是针对密切相关的菌株的。以前对不同系统发育的植物病原体丁香假单胞菌分离株的tailocin宿主范围和敏感性之间相互作用的研究表明，许多菌株具有种内tailocin活性，并且这种活性对菌株亚群具有高度精确和特异性。然而，在这里我们证明了至少一个菌株的p。由于该菌株的tailocins对其他农业上重要的植物病原体（如淀粉状葡萄球菌和过叶黄单胞菌）以及临床人类病原体肠管性霍乱沙门氏菌具有广泛的杀伤活性，因此它违背了人们的期望和目前的总体信条。此外，我们表明，这种种间杀伤活性的全谱在密切相关的菌株中并不保守，数据表明，即使tailocins可以针对不同的物种，它们的效率也不同。我们在此报告的结果强调了p的种间杀伤活性的潜力和表型差异。为进一步研究噻洛星的宿主范围和菌株特异性的进化建立平台。
Tailocins are ribosomally synthesized bacteriocins, encoded by bacterial genomes, but originally derived from bacteriophage tails. As with both bacteriocins and phage, tailocins are largely thought to be species-specific with killing activity often assumed to be directed against closely related strains. Previous investigations into interactions between tailocin host range and sensitivity across phylogenetically diverse isolates of the phytopathogenPseudomonas syringaehave demonstrated that many strains possess intraspecific tailocin activity and that this activity is highly precise and specific against subsets of strains. However, here we demonstrate that at least one strain ofP. syringae,USA011R, defies both expectations and current overarching dogma because tailocins from this strain possess broad killing activity against other agriculturally significant phytopathogens such asErwinia amylovoraandXanthomonas perforansas well as against the clinical human pathogenSalmonella entericaserovar Choleraesuis. Moreover, we show that the full spectrum of this interspecific killing activity is not conserved across closely related strains with data suggesting that even if tailocins can target different species, they do so with different efficiencies. Our results reported herein highlight the potential for and phenotypic divergence of interspecific killing activity ofP. syringaetailocins and establish a platform for further investigations into the evolution of tailocin host range and strain specificity.