Step-wise temperature decreasing cultivates a biofilm with high nitrogen removal rates at 9°C in short-term anammox biofilm tests
Step-wise temperature decreasing cultivates a biofilm with high nitrogen removal rates at 9°C in short-term anammox biofilm tests
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DOI：
10.1080/09593330.2015.1135995
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发表时间：
2016-02
影响因子：
2.8
通讯作者：
I. Zekker;E. Rikmann;A. Mandel;K. Kroon;A. Seiman;J. Mihkelson;T. Tenno;T. Tenno
中科院分区：
文献类型：
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作者：
I. Zekker;E. Rikmann;A. Mandel;K. Kroon;A. Seiman;J. Mihkelson;T. Tenno;T. Tenno
厌氧氨氧化（anammox）和亚硝化-anammox（脱氨）工艺是富氮废水处理中广泛应用的工艺。当脱氨应用向低温应用（主流废水温度较低）发展时，必须研究温度效应。在目前的研究中，在脱氨移动床生物膜反应器中，最大总氮去除率（TNRR）达到1.5 g N m−2 d−1 （0.6 kg N m−3 d−1）。温度每周逐渐降低0.5°C，在生物膜培养过程中，在15°C下保持类似的TNRR。统计分析证实，温度从20°C降至15°C不会导致不稳定。相反，在较低温度下，TNRR升高，处理效率也保持稳定。定量聚合酶链反应分析表明，尽管温度降低至15℃，布罗卡Candidatus Brocadia的厌氧氨氧化基因拷贝g−1总悬浮固体（TSS）的数量从5 × 103增加到1 × 107。TNRR的波动与进水浓度的变化密切相关。为了研究温度对TNRR的短期影响，进行了一系列批量实验，结果表明，在厌氧氨氧化温度常数（Q10）为1.3-1.6的情况下，即使在9-15°C （1.24-3.43 mg N g−1 TSS h−1）也有足够的TNRR。实验表明，与适应较高温度的生物膜相比，适应15°C的生物膜在低至9°C的温度下可以最充分地去除n。生物量适应15°C后，9°C批次试验中TNRR的下降幅度（15 - 20%）低于适应17-18°C的生物量。
ABSTRACT The anaerobic ammonium oxidation (anammox) and nitritation-anammox (deammonification) processes are widely used for N-rich wastewater treatment. When deammonification applications move towards low temperature applications (mainstream wastewater has low temperature), temperature effect has to be studied. In current research, in a deammonification moving bed biofilm reactor a maximum total nitrogen removal rate (TNRR) of 1.5 g N m−2 d−1 (0.6 kg N m−3 d−1) was achieved. Temperature was gradually lowered by 0.5°C per week, and a similar TNRR was sustained at 15°C during biofilm cultivation. Statistical analysis confirmed that a temperature decrease from 20°C down to 15° did not cause instabilities. Instead, TNRR rose and treatment efficiency remained stable at lower temperatures as well. Quantitative polymerase chain reaction analyses showed an increase in Candidatus Brocadia quantities from 5 × 103 to 1 × 107 anammox gene copies g−1 total suspended solids (TSS) despite temperature lowered to 15°C. Fluctuations in TNRR were rather related to changes in influent concentration. To study the short-term effect of temperature on the TNRR, a series of batch-scale experiments were performed which showed sufficient TNRRs even at 9–15°C (1.24–3.43 mg N g−1 TSS h−1, respectively) with anammox temperature constants (Q10) ranging 1.3–1.6. Experiments showed that a biofilm adapted to 15°C can perform N-removal most sufficiently at temperatures down to 9°C as compared with biofilm adapted to higher temperature. After biomass was adapted to 15°C, the decrease in TNRR in batch tests at 9°C was lower (15–20%) than that for biomass adapted to 17–18°C.