Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.
Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.
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发表时间：
1988-04
期刊：
影响因子：
11.2
通讯作者：
I. Chantret;A. Barbat;Elisabeth;Dussaulx;M. Brattain;A. Zweibaum
中科院分区：
文献类型：
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作者：
I. Chantret;A. Barbat;Elisabeth;Dussaulx;M. Brattain;A. Zweibaum
研究了 20 种人结肠癌细胞系发展正常肠上皮某些特征的能力，例如上皮极性、肌动蛋白结合蛋白绒毛蛋白的存在，或在标准条件下培养（如 Caco-2 细胞）或在无葡萄糖培养基中生长（如 HT-29 细胞）时肠细胞分化的发生。除了绒毛的定期存在（可被认为是细胞结肠起源的标志）外，本研究的细胞系根据其分化特征可分为四种类型。在 1 型（仅一种细胞系，即 Caco-2）中，细胞自发进行肠细胞分化，其特征是细胞层极化，形成圆顶，并存在顶刷状缘，其膜具有水解酶，如蔗糖酶-异麦芽糖酶、乳糖酶、氨肽酶 N、二肽基肽酶 IV 和碱性磷酸酶。在 2 型（三种细胞系：HT-29、HCT-EB 和 HCT-GEO）中，细胞在葡萄糖存在下生长时未分化，但在葡萄糖不存在下生长时发生肠细胞分化。在 3 型细胞系（八种细胞系：HCT-GLY、HCT-FET、HCT-FRI、HCT-CBS、HCT-ALA、Co-115、HRT-18 和 SW-1116）中，无论培养条件如何，细胞都组织成极化单层，形成圆顶，但没有任何肠细胞分化特征。在 4 型（八种细胞系：HCT-116a、HCT-R、HCT-RCA、HCT-Moser、HCT-8R、SW-480、LS-174T 和 Vaco-9P）中，无论培养条件如何，细胞都组织成多层，没有任何上皮极性或肠细胞分化特征。
Twenty human colon carcinoma cell lines were studied for their ability to develop some of the characteristics of the normal intestinal epithelium, e.g., epithelial polarity, presence of the actin-binding protein villin, or the occurrence of an enterocytic differentiation either when cultured under standard conditions, as for Caco-2 cells, or when grown in a glucose-free medium, as for HT-29 cells. Except for the regular presence of villin, which can be considered a marker of the colonic origin of the cells, the cell lines of this study could be classified into four types with regard to their differentiation characteristics. In type 1 (only one cell line, i.e., Caco-2) the cells undergo spontaneously an enterocytic differentiation characterized by a polarization of the cell layer with the formation of domes and the presence of an apical brush border the membrane of which is endowed with hydrolases such as sucrase-isomaltase, lactase, amino-peptidase N, dipeptidylpeptidase IV and alkaline phosphatase. In type 2 (three cell lines: HT-29, HCT-EB, and HCT-GEO) the cells are undifferentiated when grown in the presence of glucose but undergo an enterocytic differentiation when grown in the absence of glucose. In type 3 (eight cell lines: HCT-GLY, HCT-FET, HCT-FRI, HCT-CBS, HCT-ALA, Co-115, HRT-18, and SW-1116) the cells are organized into a polarized monolayer with the formation of domes but without any enterocytic differentiation characteristics, whatever the culture conditions. In type 4 (eight cell lines: HCT-116a, HCT-R, HCT-RCA, HCT-Moser, HCT-8R, SW-480, LS-174T, and Vaco-9P) the cells are organized into a multilayer without any feature of epithelial polarity or enterocytic differentiation, whatever the culture conditions.