Preparation and characterization of a low-phenylalanine whey hydrolysate using two-step enzymatic hydrolysis and macroporous resin adsorption
Preparation and characterization of a low-phenylalanine whey hydrolysate using two-step enzymatic hydrolysis and macroporous resin adsorption
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DOI：
10.1016/j.lwt.2020.109753
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发表时间：
2020-10-01
影响因子：
6
通讯作者：
Wu, Jianping
中科院分区：
文献类型：
--
作者：
Bu, Tingting;Zhou, Mengjie;Wu, Jianping
低苯丙氨酸（Phe）饮食对于苯丙酮尿症患者来说是必不可少的。本研究的目的是建立一种两步酶法有效地从乳清蛋白中释放苯丙氨酸，然后利用吸附剂制备低苯丙氨酸水解物（LPH）。嗜热菌蛋白酶（T）或胰酶（P）与蛋白酶A2 SD（A2）联用，水解度为42.62%~ 45.43%，水解产物中苯丙氨酸的释放率超过90%。D101大孔吸附树脂对TA 2水解液（TA 2 H）中Phe的去除率为98.38%，而活性炭对Phe的去除率为64.50%。经D101树脂柱放大后，LPH蛋白质含量为60.41g/100 g水解液，苯丙氨酸含量为1.38 ± 0.11mg/g蛋白质当量。吸附后，600 Da以下的肽含量从50.79%显著增加到93.60%，电子舌测定的苦味和涩味强度也有所增加。然而，芳香活性化合物，如米勒德反应和脂质氧化产物减少后吸收。本研究制备的LPH可作为一种合适的蛋白质替代品，用于制作苯丙酮尿症食品。
A low-phenylalanine (Phe) diet is indispensable for phenylketonuria patients. The objectives of this study were to develop a two-step enzymatic method to effectively release Phe from whey protein, and then to prepare a lowPhe hydrolysate (LPH) by using adsorbent. Thermolysin (T) or Pancreatin (P) combined with Protease A2SD (A2) showed the degree of hydrolysis ranging from 42.62% to 45.43% and released more than 90% Phe from whey protein. The D101 macroporous resin column removed 98.38% Phe from TA2 hydrolysate (TA2H), compared to 64.50% of Phe removal by activated carbon. The protein content of LPH prepared by a magnified D101 resin column was 60.41 g/100 g hydrolysate and its Phe content was 1.38 +/- 0.11 mg/g protein equivalent. After adsorption, the percentage of peptides below 600 Da significantly increased from 50.79% to 93.60%, while the intensity of bitterness and astringency measured by electronic-tongue also increased. However, aroma-active compounds such as Millard reaction and lipid oxidation products were reduced after absorption. The LPH prepared in our study could be a suitable protein substitute to make phenylketonuria food.