Distribution of Clostridium perfringens in polluted lake environments
Distribution of Clostridium perfringens in polluted lake environments
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DOI：
10.1006/anae.1997.0101
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发表时间：
1997-04-01
期刊：
影响因子：
2.3
通讯作者：
Maipa, V
中科院分区：
文献类型：
--
作者：
Bezirtzoglou, E;Panagiou, A;Maipa, V
许多与防止污染或改善淡水有关的指令也与沃茨有关，因为湖水沃茨最终继承了进入淡水的大部分污染。为了确定水深对产气荚膜梭菌的影响，我们使用了一种新的培养基，乳糖-亚硫酸盐（LS）肉汤，建议用于快速计数和鉴定产气荚膜梭菌。在污染站的以下位置中的每一个处采集重复样品：表面、60 cm、90 cm和底部（1.18cm）。使用膜过滤设备。所有样品交替地通过两个膜过滤器，第一个（20-25 μ m孔径）用于保留丰富的浮游植物，第二个（孔隙度0.45 μ m）用于C.产气荚膜杆菌将膜置于10（1）至10（4）的10倍稀释液的第一管中，并在46 ℃水浴中有氧孵育24小时。C.产气荚膜杆菌随水深而波动。营养型仅在底层取样中发现，在表层、60 cm和90 cm取样点未发现。除表面取样外，在所有取样点均发现孢子形式。产气荚膜梭菌作为一种厌氧细菌从未以营养体或孢子形式出现在表面沃茨中，即使沃茨被家庭或工业活动严重污染。营养形式只发生在底部样品，但孢子形式，更能抵抗各种环境影响发生在所有深度，除了表面。(C)北京：科学出版社.
Many of the directives that relate to the prevention of pollution or the improvement of fresh water also relate to lake waters since lake waters ultimately inherit much of the pollution that enters into fresh water. In order to determine the influence of the water depth on Clostridium perfringens, we utilised a new medium, lactose-sulfite (LS) broth, suggested for rapid enumeration and identification of C, perfringens. Duplicate samples were collected at each one of the following sites of the polluted station: surface, 60cm, 90cm and bottom (1.18cm). Membrane filtration equipment was used. All samples were alternatively passed through two membrane filters, the first (20-25 mu m pore size) was used for retention of the abundant phytoplankton and the second (porosity 0.45 mu m) for C. perfringens. Membranes were placed into the first tube of ten-fold dilutions from 10(1) to 10(4) and incubated aerobically in a waterbath at 46 degrees C for 24h. The numbers of C. perfringens fluctuated depending on the water depth. Vegetative forms were found only in the bottom sampling; they were never found in surface, 60cm and 90cm sampling sites. Sporulated forms were found in all sampling sites with the exception of the surface sampling. Clostridium perfringens as an anaerobic bacterium never occurred in the surface waters in vegetative or spore forms, even if the waters were extremely polluted by domestic or industrial activities. Vegetative forms occurred only in the bottom samples but spore forms which are more resistant to various environmental effects occurred in all depths except for the surface. (C) 1997 Academic Press.