The prevention of vocal fold scarring using autologous adipose tissue-derived stromal cells
The prevention of vocal fold scarring using autologous adipose tissue-derived stromal cells
复制标题
利用自体脂肪组织基质细胞预防声带瘢痕形成
DOI：
10.1159/000099627
复制
发表时间：
2006-01-01
影响因子：
2.7
通讯作者：
Lorenz, Robert R.
中科院分区：
文献类型：
--
作者：
Lee, Byung Joo;Wang, Soo Geun;Lorenz, Robert R.
声带疤痕和萎缩的预防和治疗仍然具有挑战性。本研究的目的是使用自体脂肪组织源性基质细胞 (ADSC) 治疗受损的声带，并评估 ADSC 在犬类动物模型中预防声带疤痕和萎缩的能力。本实验使用了十只成年狗。从所有狗的腹股沟区域脂肪组织中分离并培养 ADSC。与去端肽胶原混合后，立即使用带有 23 号针头的注射器将 ADSC (1-3 x 10(6)) 注射到每只动物的右声带中。作为对照，将去端肽胶原注射到同一只狗的左声带中。通过形态学和组织学评估来测量预防声带疤痕和萎缩的效果。 8 周时，大多数狗注射 ADSC 的一侧和对照组之间的肉芽肿和萎缩性变化存在差异。这种差异在 24 周的随访中继续存在。在组织病理学检查中，在初始治疗后 8 周，在注射 ADSC 的声带中观察到大量荧光染料标记的细胞。这项研究证明了 ADSC 在受损声带再生中的多潜能能力。将 ADSC 注射到受损的声带似乎有助于预防初始损伤后 24 周的声带疤痕和萎缩。版权所有 (c) 2007 S. Karger AG，巴塞尔。
Prevention and treatment of vocal fold scarring and atrophy remain challenging. The aim of this study was to treat injured vocal folds using autologous adipose tissue-derived stromal cells (ADSCs) and evaluate the ability to prevent vocal fold scarring and atrophy by ADSCs in a canine animal model. Ten adult dogs were used for this experiment. ADSCs from the adipose tissue from the inguinal area were isolated and cultured in all dogs. Immediately after being mixed with atelocollagen, the ADSCs (1-3 x 10(6)) were injected into the right vocal fold of each animal, using a syringe with a 23-gauge needle. As a control, atelocollagen was injected into the left vocal fold of the same dog. The effects of the prevention of vocal fold scarring and atrophy were measured by morphological and histological assessment. At 8 weeks, there was a difference in granuloma and atrophic changes between the ADSC-injected and control sides in the majority of the dogs. This difference continued to be present at the 24 weeks' follow-up. On histopathologic examination, a large number of cells labeled with a fluorochrome were observed in ADSC-injected vocal folds 8 weeks after the initial treatment. This study demonstrates the multipotential ability of ADSCs in the regeneration of injured vocal folds. Injecting ADSCs into a damaged vocal fold appears to be useful in preventing vocal fold scarring and atrophy 24 weeks after initial damage. Copyright (c) 2007 S. Karger AG, Basel.