GLYCOLYTIC-ENZYMES IN BREAST-CANCER, BENIGN BREAST DISEASE AND NORMAL BREAST-TISSUE
GLYCOLYTIC-ENZYMES IN BREAST-CANCER, BENIGN BREAST DISEASE AND NORMAL BREAST-TISSUE
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DOI：
10.1159/000217529
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
STAAL, GEJ
中科院分区：
文献类型：
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作者：
HENNIPMAN, A;SMITS, J;STAAL, GEJ
研究了乳腺癌组织中己糖激酶、磷酸果糖激酶、醛缩酶、烯醇化酶和丙酮酸激酶的活性，并与良性乳腺疾病和正常乳腺组织进行了比较。乳腺癌组织中酶活性明显高于正常乳腺组织和良性乳腺组织（P < 0.001）。良性疾病与正常相比活性的增加也具有统计学显著性（p < 0.001）。在良性疾病组中，纤维腺瘤可以与纤维囊性疾病区分开，前者通常显示出比后者更高的活性（p <0.05）。0.05）。根据其组织学分类的癌亚组无法在酶学上识别。此外，还对丙酮酸激酶和烯醇化酶的同工酶组成进行了研究。与正常乳腺组织相比，我们没有发现良性疾病中K型丙酮酸激酶表达的显著变化。纤维腺瘤与纤维囊性疾病也没有区别。然而，癌组织中K型丙酮酸激酶的量与良性疾病和正常乳腺组织相比显着升高（p < 0.001）。α的表达伽马正常乳腺组织中烯醇化酶几乎不存在。在良性疾病中，只有少数标本不显示杂交α。伽马烯醇化酶几乎所有的癌都有α。伽马烯醇化酶表达，并且在20%的癌γ中。伽马可以检测到烯醇化酶（所谓的神经元特异性烯醇化酶）。通过判别分析，给出与组织学数据相比的最佳判别的函数基于自然对数醛缩酶和γ-γ的总和。烯醇化酶亚基。与预期相反，糖酵解的常规酶;即，己糖激酶、磷酸果糖激酶和丙酮酸激酶不包括在该判别函数中。最佳拟合在良性和恶性疾病中产生90%的正确分类。如果这些发现被证实为一个更大的系列，歧视是足够强大的，形成一个临床上有用的工具的基础。
The activities of hexokinase, phosphofructokinase, aldolase, enolase and pyruvate kinase were studied in breast cancer tissues, in comparison to benign breast disease and normal breast tissues. The enzyme activities in breast cancer were significantly increased compared to normal and benign breast tissues (p < 0.001). Also the increase in activity in benign disease compared to normal was statistically significant (p < 0.001). Within the group of benign diseases, fibroadenomas could be distinguished from fibrocystic disease, the former generally showing higher activities compared to the latter (p .ltoreq. 0.05). Carcinoma subgroups, classified according to their histology, could not be recognized enzymologically. In addition, isozyme composition of pyruvate kinase and enolase was studied. We did not find a significant shift towards K type pyruvate kinase expression in benign disease compared to normal breast tissues. Also fibroadenomas did not differ from fibrocystic disease. However, the amount of K type pyruvate kinase in carcinomas proved to be significantly higher in comparison to benign disease and normal breast tissues (p < 0.001). Expression of .alpha..gamma.-enolase in normal breast tissue was virtually absent. In benign disease only a minority of specimens did not show the hybrid .alpha..gamma.-enolase. Nearly all carcinomas had .alpha..gamma.-enolase expression and in 20% of the carcinomas .gamma..gamma.-enolase could be detected (so-called neuron-specific enolase). By discriminant analysis the function giving the best discrimination compared to the histological data was based on natural logarithm aldolase and the total of .gamma.-enolase subunits. Contrary to expectation, the regular enzymes of glycolysis; i.e., hexokinase, phosphofructokinase and pyruvate kinase were not included in this discriminant function. The best fit produced a 90% correct classification in both benign and malignant disease. If these findings are confirmed to a larger series, the discrimination is sufficiently strong to form the basis of a clinically useful tool.