Inhibitory effect of phytoglycoprotein on tumor necrosis factor-α and interleukin-6 at initiation stage of colon cancer in 1,2-dimethylhydrazine-treated ICR mice
Inhibitory effect of phytoglycoprotein on tumor necrosis factor-α and interleukin-6 at initiation stage of colon cancer in 1,2-dimethylhydrazine-treated ICR mice
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DOI：
10.1016/j.taap.2007.07.010
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发表时间：
2007-12-01
影响因子：
3.8
通讯作者：
Lim, Kye-Taek
中科院分区：
文献类型：
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作者：
Lee, Sel-Jung;Lim, Kye-Taek
本研究旨在研究从榆树 (UDN) 茎中分离的植物源糖蛋白（UDN 糖蛋白，116 kDa）对 1,2-二甲基肼 (DMH) 处理的 ICR 小鼠异常隐窝病灶 (ACF) 形成的化学预防潜力。给小鼠施用 0.01% 和 0.02% 水平的 UDN 糖蛋白，持续 5 周。在UDN糖蛋白存在下，每周两次用20 mg/kg DMH处理小鼠，持续两周，并在第6周处死。我们发现UDN糖蛋白对DMH处理的小鼠中结肠异常隐窝病灶（ACF）的频率、结肠增殖细胞核抗原（PCNA）的激活和血浆乳酸脱氢酶（LDH）的释放具有抑制作用。此外，UDN糖蛋白对DMH治疗小鼠血浆硫代巴比妥酸反应物质（TBARS）的形成和血浆诱导型一氧化氮（NO）的产生具有抗氧化作用。此外，0.02% UDN 糖蛋白抑制核因子 kappa B (NF-kappa B) 和激活蛋白 1 (AP-1) 的 DNA 结合活性，同时抑制其亚基（p50、p65、c-Jun 和 c-Fos）、促炎蛋白 [诱导型一氧化氮合酶 (iNOS) 和环氧合酶 - 2 (COX-2)] 和促炎蛋白细胞因子 [肿瘤坏死因子 (TNF)-α 和白细胞介素 (IL)-6] 对 DMH 刺激的 ACF 形成的影响。根据这些结果，我们假设 UDN 糖蛋白可能有助于预防结肠癌的起始阶段。 (c) 2007 Elsevier Inc. 保留所有权利。
This study was carried out to investigate the chemopreventive potentials of plant originated glycoprotein (UDN glycoprotein, 116 kDa) isolated from the stems of Ulmus davidiana Nakai (UDN) on aberrant crypt foci (ACF) formation in 1,2-dimethylhydrazine (DMH)-treated ICR mice. UDN glycoprotein was administered to mice at 0.01% and 0.02% levels for 5 weeks. The mice were treated with 20 mg/kg DMH twice a week for 2 weeks in presence of UDN glycoprotein and killed at week 6. We found that UDN glycoprotein has inhibitory effects on the frequency of colonic aberrant crypt foci (ACF), activation of colonic proliferating cell nuclear antigen (PCNA), and release of plasma lactate dehydrogenase (LDH) in DMH-treated mice. In addition, UDN glycoprotein has anti-oxidative effects on the formation of plasma thiobarbituric acid reactive substances (TBARS) and the production of plasma inducible nitric oxide (NO) in DMH-treated mouse. Also, 0.02% UDN glycoprotein suppressed the DNA binding activities of nuclear factor-kappa B (NF-kappa B) and activator protein-1 (AP-1), accompanying the inhibitions of its subunits (p50, p65, c-Jun, and c-Fos), pro-inflammatory proteins [inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2)], and pro-inflammatory cytokines [tumor necrosis factor (TNF)-alpha and interleukin (IL)-6] on DMH-stimulated ACF formation. On the basis of these results, we assume that UDN glycoprotein may be useful for colon cancer prevention at initiation stage. (c) 2007 Elsevier Inc. All rights reserved.