Estrogenicity of xenobiotics in rainbow trout (Oncorhynchus mykiss) using in vivo synthesis of vitellogenin as a biomarker
Estrogenicity of xenobiotics in rainbow trout (Oncorhynchus mykiss) using in vivo synthesis of vitellogenin as a biomarker
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DOI：
10.1016/s0141-1136(97)00047-0
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发表时间：
1998-07-01
影响因子：
3.3
通讯作者：
Bjerregaard, P
中科院分区：
文献类型：
--
作者：
Christiansen, LB;Pedersen, KL;Bjerregaard, P
以未成熟虹鳟鱼体内卵黄蛋白原（Vtg）合成为生物标志物，研究了几种外源性物质的雌激素活性。17 β-雌二醇、己烯雌酚和炔雌醇作为阳性对照。剩余的异生物质化合物为工业壬基酚、双酚A、邻苯二甲酸丁基苄酯（BBP）和邻苯二甲酸二丁酯（DBP）。用直接夹心ELISA进行Vtg浓度的测量。17 β-雌二醇、DES和炔雌醇导致Vtg水平增加高达100 000倍。壬基酚和双酚A具有最高的外源性雌激素效力，使卵黄蛋白原水平增加约300倍，而BBP仅具有弱雌激素活性，使浓度增加约3倍。DPB不能使卵黄蛋白原浓度升高到检测限以上。(C)1998爱思唯尔科技有限公司。保留所有权利。
The estrogenicity of several xenobiotics was evaluated using in vivo vitellogenin (Vtg) synthesis lit immature rainbow trout as a biomarker. 17 beta-estradiol, DES and ethinyl estradiol were rested as positive controls. The xenobiotic compounds rested were technical nonylphenol, bisphenol A, butylbenzylphthalate (BBP) and dibutylphthalate (DBP). Measurements of the Vtg concentration was,performed with a direct sandwich ELISA. 17 beta-estradiol, DES and ethinyl estradiol caused up to 100 000-fold increases in the Vtg-levels. Nonylphenol and bisphenol A had the highest estrogenic potency of the xenobiotics increasing the vitellogenin level approximately 300-fold while BBP was only weakly estrogenic, increasing the concentration about 3 times. DPB did not raise the vitellogenin contration above the detection limit. (C) 1998 Elsevier Science Ltd. All rights reserved.