Development and optimization of a Zika virus antibody-dependent cell-mediated cytotoxicity (ADCC) assay.
Development and optimization of a Zika virus antibody-dependent cell-mediated cytotoxicity (ADCC) assay.
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DOI：
10.1016/j.jim.2020.112900
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发表时间：
2021-01
影响因子：
2.2
通讯作者：
Anderson EJ
中科院分区：
文献类型：
--
作者：
Chen X;Anderson LJ;Rostad CA;Ding L;Lai L;Mulligan M;Rouphael N;Natrajan MS;McCracken C;Anderson EJ
Zika virus (ZIKV) has become a global public health issue due to its teratogenicity and ability to cause Guillain-Barré syndrome in adults. Although anti-ZIKV envelope protein neutralizing antibodies correlate with protection, the non-neutralizing function of ZIKV antibodies including antibody-dependent cell-mediated cytotoxicity (ADCC) is incompletely understood. To study the role of ADCC antibodies during ZIKV infections, we generated a stably transfected, dual-reporter target cell line with inducible expression of a chimeric ZIKV prM-E protein on the cell surface as the target cell for the assay. By using this assay, nine of ten serum samples from ZIKV-infected patients had >20% ADCC killing of target cells, whereas none of the 12 healthy control sera had >10% ADCC killing. We also observed a time-dependent ADCC response in 2 patients with Zika. This demonstrates that this assay can detect ZIKV ADCC with high sensitivity and specificity, which could be useful for measurement of ADCC responses to ZIKV infection or vaccination.
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影响因子：
16.6
作者：
Lucas CGO;Kitoko JZ;Ferreira FM;Suzart VG;Papa MP;Coelho SVA;Cavazzoni CB;Paula-Neto HA;Olsen PC;Iwasaki A;Pereira RM;Pimentel-Coelho PM;Vale AM;de Arruda LB;Bozza MT
通讯作者：
Bozza MT
DOI：
10.1093/cid/cix732
发表时间：
2018-01-06
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Lai L;Rouphael N;Xu Y;Natrajan MS;Beck A;Hart M;Feldhammer M;Feldpausch A;Hill C;Wu H;Fairley JK;Lankford-Turner P;Kasher N;Rago P;Hu YJ;Edupuganti S;Patel SM;Murray KO;Mulligan MJ;Emory Zika Patient Study Team
通讯作者：
Emory Zika Patient Study Team
影响因子：
3.1
作者：
Charoensri, Nicha;Suphatrakul, Amporn;Sittisombut, Nopporn
通讯作者：
Sittisombut, Nopporn
影响因子：
5.4
作者：
Frenzel, K.;Lehmann, J.;Hofmann, J.
通讯作者：
Hofmann, J.
DOI：
10.3390/v10090480
发表时间：
2018-09-07
期刊：
Viruses
影响因子：
--
作者：
Delgado FG;Torres KI;Castellanos JE;Romero-Sánchez C;Simon-Lorière E;Sakuntabhai A;Roth C
通讯作者：
Roth C