Fatty acids isolated from royal jelly modulate dendritic cell-mediated immune response in vitro
Fatty acids isolated from royal jelly modulate dendritic cell-mediated immune response in vitro
复制标题
DOI：
10.1016/j.intimp.2007.05.005
复制
发表时间：
2007-09-01
影响因子：
5.6
通讯作者：
Colic, Miodrag
中科院分区：
文献类型：
--
作者：
Vucevic, Dragana;Melliou, Eleni;Colic, Miodrag
皇家果冻（RJ），特别是其蛋白质成分，已被证明具有免疫调节活性。然而，几乎没有人知道RJ脂肪酸对免疫系统的影响。在这项工作中，我们研究了10-羟基-2-癸酸（10-HDA）和3，10-二羟基癸酸（3，10-DDA），分离自RJ，对免疫反应的影响，使用大鼠树突状细胞（DC）-T细胞共培养模型。这两种脂肪酸，在较高浓度下，抑制同种异体T细胞的增殖。10-HDA的作用更强，其次是减少白细胞介素-2（IL-2）的产生和下调IL-2受体的表达。用10 μ g/ml脂肪酸培养的脾DC下调CD 86的表达和IL-12的产生，但上调IL-10的产生。与此相反，DC，用100 μ g/ml的3，10-DDA预处理，上调CD 86的表达和增强同种异体T细胞的增殖。两种脂肪酸的最高剂量（200 μ g/ml）对T细胞和DC均不凋亡，下调MHC II类和CD 86的表达，降低IL-12的产生，并使这些DC的同种异体刺激性降低。还使用大鼠锁孔淋巴管血蓝蛋白免疫模型在体内证实了3，10-DDA的免疫抑制活性。总之，我们的研究结果显示了RJ脂肪酸的免疫调节活性，并表明DC是其作用的重要靶点。(c)2007 Elsevier B. V.保留所有权利。
Royal jelly (RJ), especially its protein components, has been shown to possess immunomodulatory activity. However, almost nothing is known about the influence of RJ fatty acids on the immune system. In this work we studied the effect of 10-hydroxy-2-decanoic acid (10-HDA) and 3,10-dihydroxy-decanoic acid (3,1 0-DDA), isolated from RJ, on the immune response using a model of rat dendritic cell (DC)-T-cell cocultures. Both fatty acids, at higher concentrations, inhibited the proliferation of allogeneic T cells. The effect of 10-HDA was stronger and was followed by a decrease in interleukin-2 (IL-2) production and down-regulation of IL-2 receptor expression. Spleen DC, cultivated with 10 mu g/ml of fatty acids down-regulated the expression of CD86 and the production of IL-12, but up-regulated the production of IL-10. In contrast, DC, pretreated with 100 mu g/ml of 3,10-DDA, up-regulated the expression of CD86 and augmented the proliferation of allogeneic T cells. The highest dose (200 mu g/ml) of both fatty acids which was non-apoptotic for both T cells and DC, down-regulated the expression of MHC class II and CD86, decreased the production of IL-12 and made these DC less allostimulatory. The immunosuppressive activity of 3,10-DDA was also confirmed in vivo, using a model of Keyhole lymphet hemocyanine immunization of rats. In conclusion, our results showed the immunomodulatory activity of RJ fatty acids and suggest that DC are a significant target of their action. (c) 2007 Elsevier B.V. All rights reserved.